pubmed-article:9511743 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:9511743 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
pubmed-article:9511743 | lifeskim:mentions | umls-concept:C0067966 | lld:lifeskim |
pubmed-article:9511743 | lifeskim:mentions | umls-concept:C1334043 | lld:lifeskim |
pubmed-article:9511743 | lifeskim:mentions | umls-concept:C0086149 | lld:lifeskim |
pubmed-article:9511743 | pubmed:issue | 1 | lld:pubmed |
pubmed-article:9511743 | pubmed:dateCreated | 1998-4-7 | lld:pubmed |
pubmed-article:9511743 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:9511743 | pubmed:abstractText | The N-ethylmaleimide-sensitive fusion protein (NSF) is required for vesicular membrane fusion in multiple cellular functions. We have cloned a cDNA encoding the Dictyostelium discoideum homolog of the NSF protein. This cDNA hybridizes with a single fragment in Southern blots suggesting that NSF is encoded by a single gene in the amoeba. It is expressed constitutively during vegetative growth and throughout the differentiation cycle. The encoded gene product comprises 738 aa with a predicted molecular mass of 82 kDa. It shows the characteristic three-domain structure of NSF proteins. A more divergent amino-terminal part is followed by two highly conserved ATP-binding domains featuring Walker A and B signature sequences. The D. discoideum protein presents an overall aa sequence identity of 44% when compared to known NSF homologs. The monoclonal antibody 2E5 directed against Cricetellus griseus NSF recognizes a protein with a molecular weight of approx. 80 000 in a D. discoideum crude extract and the recombinant D. discoideum His6-NSF expressed in Escherichia coli. | lld:pubmed |
pubmed-article:9511743 | pubmed:language | eng | lld:pubmed |
pubmed-article:9511743 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:9511743 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:9511743 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:9511743 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:9511743 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:9511743 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:9511743 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:9511743 | pubmed:month | Jan | lld:pubmed |
pubmed-article:9511743 | pubmed:issn | 0378-1119 | lld:pubmed |
pubmed-article:9511743 | pubmed:author | pubmed-author:SatreMM | lld:pubmed |
pubmed-article:9511743 | pubmed:author | pubmed-author:BruckertFF | lld:pubmed |
pubmed-article:9511743 | pubmed:author | pubmed-author:WeidenhauptMM | lld:pubmed |
pubmed-article:9511743 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:9511743 | pubmed:day | 19 | lld:pubmed |
pubmed-article:9511743 | pubmed:volume | 207 | lld:pubmed |
pubmed-article:9511743 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:9511743 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:9511743 | pubmed:pagination | 53-60 | lld:pubmed |
pubmed-article:9511743 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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pubmed-article:9511743 | pubmed:year | 1998 | lld:pubmed |
pubmed-article:9511743 | pubmed:articleTitle | Identification of the Dictyostelium discoideum homolog of the N-ethylmaleimide-sensitive fusion protein. | lld:pubmed |
pubmed-article:9511743 | pubmed:affiliation | Laboratoire de Biochimie et Biochimie et Biophysique des Systèmes Intégrés, UMR 314 CEA-CNRS, CEA-Grenoble, France. | lld:pubmed |
pubmed-article:9511743 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:9511743 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9511743 | lld:pubmed |