| pubmed-article:9485376 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9485376 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9485376 | lifeskim:mentions | umls-concept:C0086661 | lld:lifeskim |
| pubmed-article:9485376 | lifeskim:mentions | umls-concept:C0033713 | lld:lifeskim |
| pubmed-article:9485376 | lifeskim:mentions | umls-concept:C2243187 | lld:lifeskim |
| pubmed-article:9485376 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:9485376 | pubmed:dateCreated | 1998-3-20 | lld:pubmed |
| pubmed-article:9485376 | pubmed:abstractText | Triplex-forming oligonucleotides (TFOs) have been shown to inhibit both transcription in vitro and the expression of target genes in cell culture by binding to polypurine/polypyrimidine sequences in several human gene promoters. The c-myc protooncogene is overexpressed in a variety of human cancers and appears to play an important role in the proliferation of these cells. In an attempt to assay the ability of triplex-forming oligonucleotides to inhibit expression of a target gene in vivo, we have developed a cellular system involving transfection of a c-myc promoter-driven luciferase reporter plasmid with triplex-forming oligonucleotides targeted to the human c-myc protooncogene. To increase the stability of the TFO, we have used modified phosphorothioate oligonucleotides. Triplex formation with a modified phosphorothioate oligonucleotide occurs with approximately equal binding affinity as that seen using a phosphodiester oligonucleotide. Phosphorothioate-modified TFOs targeted to c-myc inhibit transcription of the c-myc promoter in HeLa cells as demonstrated by a decrease in luciferase expression from a luciferase reporter gene construct. These results suggests that triplex formation may represent a gene-specific means of inhibiting specific protooncogene expression. | lld:pubmed |
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| pubmed-article:9485376 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9485376 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9485376 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9485376 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9485376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9485376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9485376 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9485376 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:9485376 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:9485376 | pubmed:author | pubmed-author:MillerD MDM | lld:pubmed |
| pubmed-article:9485376 | pubmed:author | pubmed-author:ThomasSS | lld:pubmed |
| pubmed-article:9485376 | pubmed:author | pubmed-author:LamK MKM | lld:pubmed |
| pubmed-article:9485376 | pubmed:author | pubmed-author:MayfieldCC | lld:pubmed |
| pubmed-article:9485376 | pubmed:author | pubmed-author:VigneswaranNN | lld:pubmed |
| pubmed-article:9485376 | pubmed:author | pubmed-author:EbbinghausSS | lld:pubmed |
| pubmed-article:9485376 | pubmed:author | pubmed-author:JonesD EDEJr | lld:pubmed |
| pubmed-article:9485376 | pubmed:author | pubmed-author:ReddochJ FJF | lld:pubmed |
| pubmed-article:9485376 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9485376 | pubmed:day | 24 | lld:pubmed |
| pubmed-article:9485376 | pubmed:volume | 37 | lld:pubmed |
| pubmed-article:9485376 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9485376 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9485376 | pubmed:pagination | 2299-304 | lld:pubmed |
| pubmed-article:9485376 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:9485376 | pubmed:meshHeading | pubmed-meshheading:9485376-... | lld:pubmed |
| pubmed-article:9485376 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9485376 | pubmed:articleTitle | Inhibition of transcription of the human c-myc protooncogene by intermolecular triplex. | lld:pubmed |
| pubmed-article:9485376 | pubmed:affiliation | Department of Biochemistry, University of Alabama at Birmingham, Birmingham, Alabama 35294-0001, USA. | lld:pubmed |
| pubmed-article:9485376 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9485376 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9485376 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:9485376 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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