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pubmed-article:9457074pubmed:abstractTextDNA-protein cross-linkages were performed in intact undifferentiated and differentiated-HL60 cells by the action of cis-diammine dichloroplatinum. Total nuclear matrix proteins and DNA cross-linked nuclear matrix proteins were resolved by two-dimensional gel electrophoresis. The comparison of the electrophoretic patterns allowed the identification of a set of differentiation-induced nuclear matrix proteins cross-linked to DNA. One of these proteins binds cloned histone SAR sequences. Our results outline an experimental strategy for isolating and characterizing nuclear matrix components that may play a fundamental role in the overall control and coordination of gene expression during differentiation.lld:pubmed
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pubmed-article:9457074pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:9457074pubmed:articleTitleDifferentiation-specific nuclear matrix proteins cross-linked to DNA by cis-diammine dichloroplatinum.lld:pubmed
pubmed-article:9457074pubmed:affiliationMicrobiology Institute Faculty of Medicine and Surgery, University of Rome La Sapienza, Italy. Mattia@axrma.uniroma1.itlld:pubmed
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