| pubmed-article:9442926 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9442926 | lifeskim:mentions | umls-concept:C0024141 | lld:lifeskim |
| pubmed-article:9442926 | lifeskim:mentions | umls-concept:C0007004 | lld:lifeskim |
| pubmed-article:9442926 | lifeskim:mentions | umls-concept:C0002198 | lld:lifeskim |
| pubmed-article:9442926 | lifeskim:mentions | umls-concept:C0442805 | lld:lifeskim |
| pubmed-article:9442926 | lifeskim:mentions | umls-concept:C0332281 | lld:lifeskim |
| pubmed-article:9442926 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:9442926 | pubmed:dateCreated | 1998-2-20 | lld:pubmed |
| pubmed-article:9442926 | pubmed:abstractText | Using lectin blots in conjunction with peptide mapping, alpha 2-macroglobulin micropurified from systemic lupus erythematosus (SLE) patients was shown to become abnormally glycosylated suggesting the occurrence of complex glycosylation in this pathological condition. To confirm there is indeed a quantitative increase in specific monosaccharides in this protein; alpha 2-macroglobulin was micropurified from a battery of 37 serum samples which included 6 normal donors (3 male and 3 female), 23 SLE patients, 6 rheumatoid arthritis patients, 1 mixed connective tissue disease patient, and 1 Sjogren's syndrome patient; for carbohydrate analysis. It was noted that the concentration of total monosaccharides in alpha 2-macroglobulin micropurified from serum samples of SLE patients is significantly higher than normal donors with a mean +/- SD of 188 +/- 410 micrograms/mg protein (SLE, n = 23) versus 14.5 +/- 4 micrograms/mg protein (normal, n = 6) even though there was a high variation in the level of monosaccharides among the SLE patients. An increase in oligosaccharides in alpha 2-macroglobulin from SLE patients compared to normal subjects was confirmed by concanavalin A (Con A) blots using peptide fragments derived from the micropurified protein. Since the interaction of peptide fragments derived from alpha 2-macroglobulin with Con A requires the presence of mannose and/or glucose residues, we have also examined if there are any correlations between the levels of mannose and glucose in alpha 2-macroglobulin and SLE. The concentration of mannose (38 +/- 60 micrograms/mg protein) in alpha 2-macroglobulin derived from SLE patients was significantly higher than normal donors (mannose, 4.8 +/- 1 micrograms/mg protein) however, the concentration of glucose in alpha 2-macroglobulin derived from SLE patients when compared to normal donors was not statistically significant, 18 +/- 20 micrograms/mg protein in SLE versus 2 +/- 0.5 micrograms/mg protein in normal donors due to high variation between samples. Also, the concentration of galactose in alpha 2-macroglobulin from SLE patients was significantly higher than normal donors (45.7 +/- 173 micrograms/mg protein versus 0.13 +/- 0.03 microgram/mg protein). These results illustrate quantification of carbohydrate in selected glycoproteins such as alpha 2-macroglobulin may be a novel and alternative clinical marker for SLE. | lld:pubmed |
| pubmed-article:9442926 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9442926 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9442926 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9442926 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9442926 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9442926 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9442926 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9442926 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9442926 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9442926 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9442926 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9442926 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9442926 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9442926 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:9442926 | pubmed:issn | 1039-9712 | lld:pubmed |
| pubmed-article:9442926 | pubmed:author | pubmed-author:LahitaRR | lld:pubmed |
| pubmed-article:9442926 | pubmed:author | pubmed-author:SilvestriniBB | lld:pubmed |
| pubmed-article:9442926 | pubmed:author | pubmed-author:MaM CMC | lld:pubmed |
| pubmed-article:9442926 | pubmed:author | pubmed-author:ChengC YCY | lld:pubmed |
| pubmed-article:9442926 | pubmed:author | pubmed-author:PanzironiCC | lld:pubmed |
| pubmed-article:9442926 | pubmed:author | pubmed-author:MrukDD | lld:pubmed |
| pubmed-article:9442926 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9442926 | pubmed:volume | 43 | lld:pubmed |
| pubmed-article:9442926 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9442926 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9442926 | pubmed:pagination | 1305-22 | lld:pubmed |
| pubmed-article:9442926 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9442926 | pubmed:meshHeading | pubmed-meshheading:9442926-... | lld:pubmed |
| pubmed-article:9442926 | pubmed:meshHeading | pubmed-meshheading:9442926-... | lld:pubmed |
| pubmed-article:9442926 | pubmed:meshHeading | pubmed-meshheading:9442926-... | lld:pubmed |
| pubmed-article:9442926 | pubmed:meshHeading | pubmed-meshheading:9442926-... | lld:pubmed |
| pubmed-article:9442926 | pubmed:meshHeading | pubmed-meshheading:9442926-... | lld:pubmed |
| pubmed-article:9442926 | pubmed:meshHeading | pubmed-meshheading:9442926-... | lld:pubmed |
| pubmed-article:9442926 | pubmed:meshHeading | pubmed-meshheading:9442926-... | lld:pubmed |
| pubmed-article:9442926 | pubmed:meshHeading | pubmed-meshheading:9442926-... | lld:pubmed |
| pubmed-article:9442926 | pubmed:meshHeading | pubmed-meshheading:9442926-... | lld:pubmed |
| pubmed-article:9442926 | pubmed:meshHeading | pubmed-meshheading:9442926-... | lld:pubmed |
| pubmed-article:9442926 | pubmed:meshHeading | pubmed-meshheading:9442926-... | lld:pubmed |
| pubmed-article:9442926 | pubmed:meshHeading | pubmed-meshheading:9442926-... | lld:pubmed |
| pubmed-article:9442926 | pubmed:meshHeading | pubmed-meshheading:9442926-... | lld:pubmed |
| pubmed-article:9442926 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9442926 | pubmed:articleTitle | An increase in the carbohydrate moiety of alpha 2-macroglobulin is associated with systemic lupus erythematosus (SLE). | lld:pubmed |
| pubmed-article:9442926 | pubmed:affiliation | The Population Council, New York, NY 10021, USA. | lld:pubmed |
| pubmed-article:9442926 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9442926 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
