| pubmed-article:9430666 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9430666 | lifeskim:mentions | umls-concept:C0752065 | lld:lifeskim |
| pubmed-article:9430666 | lifeskim:mentions | umls-concept:C0441471 | lld:lifeskim |
| pubmed-article:9430666 | lifeskim:mentions | umls-concept:C0332466 | lld:lifeskim |
| pubmed-article:9430666 | lifeskim:mentions | umls-concept:C1522290 | lld:lifeskim |
| pubmed-article:9430666 | lifeskim:mentions | umls-concept:C0169268 | lld:lifeskim |
| pubmed-article:9430666 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:9430666 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:9430666 | lifeskim:mentions | umls-concept:C1522240 | lld:lifeskim |
| pubmed-article:9430666 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:9430666 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:9430666 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:9430666 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:9430666 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:9430666 | pubmed:dateCreated | 1998-2-12 | lld:pubmed |
| pubmed-article:9430666 | pubmed:abstractText | The N-ethylmaleimide-sensitive factor (NSF) is required for multiple intracellular vesicle transport events. In vitro biochemical studies have demonstrated that NSF, soluble NSF attachment proteins (SNAPs), and SNAP receptors from a 20 S particle. This complex is disassembled by the ATPase activity of NSF. We have studied particle disassembly in a membrane environment by examining the binding of recombinant SNAPs and NSF to endosomal membranes. We present evidence that alpha-SNAP is released from the membranes in a temperature- and time-dependent manner and that this release is mediated by the ATPase activity of NSF. Our results indicate that NSF mutants in the first ATP binding domain completely abrogate alpha-SNAP release, whereas no inhibitory effect is observed with a mutant in the second ATP binding domain. Interestingly, neither beta-SNAP nor gamma-SNAP are released by the ATPase activity of NSF, indicating that these proteins are retained on the membranes by interactions that differ from those that retain alpha-SNAP. Although the small Rab GTPases are known to play a role in SNARE complex assembly, our results indicate that these GTPases do not regulate the NSF-dependent release of alpha-SNAP. | lld:pubmed |
| pubmed-article:9430666 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9430666 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9430666 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9430666 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9430666 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9430666 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9430666 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9430666 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9430666 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9430666 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9430666 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9430666 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9430666 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9430666 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:9430666 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:9430666 | pubmed:author | pubmed-author:StahlP DPD | lld:pubmed |
| pubmed-article:9430666 | pubmed:author | pubmed-author:WhiteheartS... | lld:pubmed |
| pubmed-article:9430666 | pubmed:author | pubmed-author:ColomboM IMI | lld:pubmed |
| pubmed-article:9430666 | pubmed:author | pubmed-author:GelbermanS... | lld:pubmed |
| pubmed-article:9430666 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9430666 | pubmed:day | 16 | lld:pubmed |
| pubmed-article:9430666 | pubmed:volume | 273 | lld:pubmed |
| pubmed-article:9430666 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9430666 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9430666 | pubmed:pagination | 1334-8 | lld:pubmed |
| pubmed-article:9430666 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9430666 | pubmed:meshHeading | pubmed-meshheading:9430666-... | lld:pubmed |
| pubmed-article:9430666 | pubmed:meshHeading | pubmed-meshheading:9430666-... | lld:pubmed |
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| pubmed-article:9430666 | pubmed:meshHeading | pubmed-meshheading:9430666-... | lld:pubmed |
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| pubmed-article:9430666 | pubmed:meshHeading | pubmed-meshheading:9430666-... | lld:pubmed |
| pubmed-article:9430666 | pubmed:meshHeading | pubmed-meshheading:9430666-... | lld:pubmed |
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| pubmed-article:9430666 | pubmed:meshHeading | pubmed-meshheading:9430666-... | lld:pubmed |
| pubmed-article:9430666 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9430666 | pubmed:articleTitle | N-ethylmaleimide-sensitive factor-dependent alpha-SNAP release, an early event in the docking/fusion process, is not regulated by Rab GTPases. | lld:pubmed |
| pubmed-article:9430666 | pubmed:affiliation | Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA. | lld:pubmed |
| pubmed-article:9430666 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9430666 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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