| pubmed-article:9415710 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9415710 | lifeskim:mentions | umls-concept:C0027713 | lld:lifeskim |
| pubmed-article:9415710 | lifeskim:mentions | umls-concept:C0205107 | lld:lifeskim |
| pubmed-article:9415710 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:9415710 | lifeskim:mentions | umls-concept:C2003941 | lld:lifeskim |
| pubmed-article:9415710 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:9415710 | lifeskim:mentions | umls-concept:C0678640 | lld:lifeskim |
| pubmed-article:9415710 | lifeskim:mentions | umls-concept:C0597484 | lld:lifeskim |
| pubmed-article:9415710 | lifeskim:mentions | umls-concept:C0449560 | lld:lifeskim |
| pubmed-article:9415710 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:9415710 | pubmed:dateCreated | 1998-1-29 | lld:pubmed |
| pubmed-article:9415710 | pubmed:abstractText | Activation of alpha 1-adrenergic receptors (alpha 1-AR) increases Na+/H+ exchange (NHE) in proximal tubule. NHE mediates the majority of active Na+ absorption in the proximal tubule. Three alpha 1-AR subtypes have been detected in kidney by molecular and binding techniques. We detected message for all three alpha 1-AR subtypes in mouse proximal tubule cells through reverse transcription-polymerase chain reaction and Northern analysis. To determine the alpha 1-AR subtypes that regulate NHE in mouse proximal tubule cells, two strategies were used: (i) antisense oligodeoxynucleotides (ODNs) to selectively inhibit expression of alpha 1A-, alpha 1B-, and alpha 1D-AR subtypes and (ii) subtype-selective alpha 1-AR antagonists. Streptolysin-O permeabilization was used to introduce antisense and sense ODNs into cells three times over 72 hr. Western blot analysis of membranes prepared from cells treated with alpha 1B-AR antisense ODN demonstrated that alpha 1B-AR protein expression was reduced by 90% at 72 hr compared with control or sense ODN treatments. Functional regulation of NHE by alpha 1-ARs was determined by alpha 1-AR agonist changes in intracellular pH (pHi) in cells grown on coverslips and loaded with 2',7'-bis(2-carboxyethyl)-5(6)carboxyfluorescein-acetoxymethyl ester. Antisense ODNs for alpha 1B-AR significantly reduced phenylephrine (PHE)-induced maximal changes in pHi by 49%. The PHE-induced changes in pHi observed in cells treated with alpha 1A-AR antisense ODNs was reduced by 42%. The selective alpha 1A-AR antagonist WB-4101 and the alpha 1B-AR antagonist spiperone reduce PHE-induced pHi increases to a comparable extent. No significant changes in pHi were observed with cells treated with alpha 1D-AR antisense ODNs or the alpha 1D-AR antagonist BMY 7378 compared with untreated cells. Combined treatment with alpha 1A- and alpha 1B-AR antisense ODNs and antagonists additively inhibits PHE-induced delta pHi by 90%. We conclude that alpha 1A and alpha 1B-AR but not alpha 1D-ARs regulate NHE in proximal tubule cells. | lld:pubmed |
| pubmed-article:9415710 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9415710 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9415710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9415710 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9415710 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:9415710 | pubmed:issn | 0026-895X | lld:pubmed |
| pubmed-article:9415710 | pubmed:author | pubmed-author:FriedmanP APA | lld:pubmed |
| pubmed-article:9415710 | pubmed:author | pubmed-author:LisHH | lld:pubmed |
| pubmed-article:9415710 | pubmed:author | pubmed-author:DreznerM KMK | lld:pubmed |
| pubmed-article:9415710 | pubmed:author | pubmed-author:NesbittTT | lld:pubmed |
| pubmed-article:9415710 | pubmed:author | pubmed-author:GesekF AFA | lld:pubmed |
| pubmed-article:9415710 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9415710 | pubmed:volume | 52 | lld:pubmed |
| pubmed-article:9415710 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9415710 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9415710 | pubmed:pagination | 1010-8 | lld:pubmed |
| pubmed-article:9415710 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:9415710 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9415710 | pubmed:articleTitle | Proximal nephron Na+/H+ exchange is regulated by alpha 1A- and alpha 1B-adrenergic receptor subtypes. | lld:pubmed |
| pubmed-article:9415710 | pubmed:affiliation | Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA. | lld:pubmed |
| pubmed-article:9415710 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9415710 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9415710 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9415710 | lld:pubmed |
