| pubmed-article:9407314 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9407314 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9407314 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:9407314 | lifeskim:mentions | umls-concept:C0037659 | lld:lifeskim |
| pubmed-article:9407314 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:9407314 | lifeskim:mentions | umls-concept:C0021017 | lld:lifeskim |
| pubmed-article:9407314 | lifeskim:mentions | umls-concept:C0442805 | lld:lifeskim |
| pubmed-article:9407314 | lifeskim:mentions | umls-concept:C1539798 | lld:lifeskim |
| pubmed-article:9407314 | lifeskim:mentions | umls-concept:C2754073 | lld:lifeskim |
| pubmed-article:9407314 | lifeskim:mentions | umls-concept:C1514485 | lld:lifeskim |
| pubmed-article:9407314 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:9407314 | pubmed:dateCreated | 1998-1-23 | lld:pubmed |
| pubmed-article:9407314 | pubmed:abstractText | The neuropeptide somatostatin (SRIF) modulates normal and leukemia T cell proliferation. However, neither molecular isotypes of receptors nor mechanisms involved in these somatostatin actions have been elucidated as yet. Here we show by using RT-PCR approach that mitogen-activated leukemia T cells (Jurkat) express mRNA for a single somatostatin receptor, sst3. This mRNA is apparently translated into protein since specific somatostatin binding sites (K11 = 78 +/- 3 pM) were detected in semipurified plasma membrane preparations by using 125I-Tyr1-SRIF14 as a radioligand. Moreover, somatostatin inhibits adenylyl cyclase activity with similar efficiency (IC50 = 23 +/- 4 pM) thus strongly suggesting a functional coupling of sst3 receptor to this transduction pathway. The involvement of sst3 receptor in immuno-modulatory actions of somatostatin was assessed by analysis of neuropeptide effects on IL-2 secretion and on proliferation of mitogen-activated Jurkat cells. Our data show that in the concentrations comprised between 10 pM and 10 nM, somatostatin potentiates IL-2 secretion. This effect is correlated with somatostatin-dependent increase of Jurkat cell proliferation since the EC50 concentrations for both actions were almost identical (EC50 = 22 +/- 9 pM and EC50 = 12 +/- 1 pM for IL-2 secretion and proliferation, respectively). Altogether, these data strongly suggest that in mitogen-activated Jurkat cells, somatostatin increases cell proliferation through the increase of IL-2 secretion via a functional sst3 receptor negatively coupled to the adenylyl cyclase pathway. | lld:pubmed |
| pubmed-article:9407314 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9407314 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9407314 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9407314 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9407314 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9407314 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9407314 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9407314 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9407314 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9407314 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9407314 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9407314 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9407314 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:9407314 | pubmed:issn | 0730-2312 | lld:pubmed |
| pubmed-article:9407314 | pubmed:author | pubmed-author:EnjalbertAA | lld:pubmed |
| pubmed-article:9407314 | pubmed:author | pubmed-author:CardosoAA | lld:pubmed |
| pubmed-article:9407314 | pubmed:author | pubmed-author:GautronJ PJP | lld:pubmed |
| pubmed-article:9407314 | pubmed:author | pubmed-author:HorvatBB | lld:pubmed |
| pubmed-article:9407314 | pubmed:author | pubmed-author:GautierNN | lld:pubmed |
| pubmed-article:9407314 | pubmed:author | pubmed-author:KranticSS | lld:pubmed |
| pubmed-article:9407314 | pubmed:author | pubmed-author:el GhamrawyCC | lld:pubmed |
| pubmed-article:9407314 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9407314 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:9407314 | pubmed:volume | 68 | lld:pubmed |
| pubmed-article:9407314 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9407314 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9407314 | pubmed:pagination | 62-73 | lld:pubmed |
| pubmed-article:9407314 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9407314 | pubmed:meshHeading | pubmed-meshheading:9407314-... | lld:pubmed |
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| pubmed-article:9407314 | pubmed:meshHeading | pubmed-meshheading:9407314-... | lld:pubmed |
| pubmed-article:9407314 | pubmed:meshHeading | pubmed-meshheading:9407314-... | lld:pubmed |
| pubmed-article:9407314 | pubmed:meshHeading | pubmed-meshheading:9407314-... | lld:pubmed |
| pubmed-article:9407314 | pubmed:meshHeading | pubmed-meshheading:9407314-... | lld:pubmed |
| pubmed-article:9407314 | pubmed:meshHeading | pubmed-meshheading:9407314-... | lld:pubmed |
| pubmed-article:9407314 | pubmed:meshHeading | pubmed-meshheading:9407314-... | lld:pubmed |
| pubmed-article:9407314 | pubmed:meshHeading | pubmed-meshheading:9407314-... | lld:pubmed |
| pubmed-article:9407314 | pubmed:meshHeading | pubmed-meshheading:9407314-... | lld:pubmed |
| pubmed-article:9407314 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9407314 | pubmed:articleTitle | Somatostatin increases mitogen-induced IL-2 secretion and proliferation of human Jurkat T cells via sst3 receptor isotype. | lld:pubmed |
| pubmed-article:9407314 | pubmed:affiliation | Laboratoire de Biologie Moléculaire et Cellulaire, Lyon, France. | lld:pubmed |
| pubmed-article:9407314 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9407314 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9407314 | lld:pubmed |
