| pubmed-article:9404420 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9404420 | lifeskim:mentions | umls-concept:C0599779 | lld:lifeskim |
| pubmed-article:9404420 | lifeskim:mentions | umls-concept:C0026844 | lld:lifeskim |
| pubmed-article:9404420 | lifeskim:mentions | umls-concept:C0015980 | lld:lifeskim |
| pubmed-article:9404420 | lifeskim:mentions | umls-concept:C0340652 | lld:lifeskim |
| pubmed-article:9404420 | lifeskim:mentions | umls-concept:C1658578 | lld:lifeskim |
| pubmed-article:9404420 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:9404420 | lifeskim:mentions | umls-concept:C1517499 | lld:lifeskim |
| pubmed-article:9404420 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:9404420 | pubmed:dateCreated | 1998-1-6 | lld:pubmed |
| pubmed-article:9404420 | pubmed:abstractText | Vascular hypertrophy may increase the blood pressure by its effect on vascular resistance. In this study, adenoviral gene transfer of IFN-beta was analysed in a porcine model of balloon injury to determine whether a secreted growth inhibitory protein might affect the regrowth of vascular smooth muscle cells (VSMC) in vitro and in arteries. An adenoviral vector encoding IFN-beta (ADV-IFN-beta) was constructed by homologous recombination between sub360 genomic DNA, an ADV 5 derivative with a deletion in the E3 region and a porcine IFN-beta expression plasmid. Its antiproliferative effect was analysed using cell proliferation assays, and used in a porcine model of balloon injury. After injury, arteries were immediately transfected with 7 x 10(9) plaques forming units of either ADV-IFN-beta or a control E1A deficient adenovirus that does not encode a recombinant protein, ADV-delta E1. The intima/media (I/M) area ratio was determined by quantitative morphometry 21 days after artery injury and gene transfer. Expression of recombinant porcine IFN-beta in VSMC reduced cell proliferation significantly in vitro, and supernatants derived from IFN-beta vector infected cells inhibited VSMC proliferation relative to controls. When introduced into porcine arteries after balloon injury, a reduction in I/M ratio of 30% was found. I/M ratio in the IFN-beta transduced arteries was 0.54 +/- 0.03 vs 0.69 +/- 0.06 in ADV-delta E1 transfected arteries and 0.702 +/- 0.05 in the non-transfected arteries. Gene transfer of an adenoviral vector encoding IFN-beta to VSMC and injured arteries reduced cell proliferation and vascular thickening. This approach is potentially applicable to vascular proliferative diseases. | lld:pubmed |
| pubmed-article:9404420 | pubmed:language | fre | lld:pubmed |
| pubmed-article:9404420 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9404420 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9404420 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9404420 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9404420 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:9404420 | pubmed:issn | 0003-9683 | lld:pubmed |
| pubmed-article:9404420 | pubmed:author | pubmed-author:SasMM | lld:pubmed |
| pubmed-article:9404420 | pubmed:author | pubmed-author:GordonDD | lld:pubmed |
| pubmed-article:9404420 | pubmed:author | pubmed-author:StephanDD | lld:pubmed |
| pubmed-article:9404420 | pubmed:author | pubmed-author:NabelG JGJ | lld:pubmed |
| pubmed-article:9404420 | pubmed:author | pubmed-author:NabelE GEG | lld:pubmed |
| pubmed-article:9404420 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9404420 | pubmed:volume | 90 | lld:pubmed |
| pubmed-article:9404420 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9404420 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9404420 | pubmed:pagination | 1121-5 | lld:pubmed |
| pubmed-article:9404420 | pubmed:dateRevised | 2009-2-13 | lld:pubmed |
| pubmed-article:9404420 | pubmed:meshHeading | pubmed-meshheading:9404420-... | lld:pubmed |
| pubmed-article:9404420 | pubmed:meshHeading | pubmed-meshheading:9404420-... | lld:pubmed |
| pubmed-article:9404420 | pubmed:meshHeading | pubmed-meshheading:9404420-... | lld:pubmed |
| pubmed-article:9404420 | pubmed:meshHeading | pubmed-meshheading:9404420-... | lld:pubmed |
| pubmed-article:9404420 | pubmed:meshHeading | pubmed-meshheading:9404420-... | lld:pubmed |
| pubmed-article:9404420 | pubmed:meshHeading | pubmed-meshheading:9404420-... | lld:pubmed |
| pubmed-article:9404420 | pubmed:meshHeading | pubmed-meshheading:9404420-... | lld:pubmed |
| pubmed-article:9404420 | pubmed:meshHeading | pubmed-meshheading:9404420-... | lld:pubmed |
| pubmed-article:9404420 | pubmed:meshHeading | pubmed-meshheading:9404420-... | lld:pubmed |
| pubmed-article:9404420 | pubmed:meshHeading | pubmed-meshheading:9404420-... | lld:pubmed |
| pubmed-article:9404420 | pubmed:meshHeading | pubmed-meshheading:9404420-... | lld:pubmed |
| pubmed-article:9404420 | pubmed:meshHeading | pubmed-meshheading:9404420-... | lld:pubmed |
| pubmed-article:9404420 | pubmed:meshHeading | pubmed-meshheading:9404420-... | lld:pubmed |
| pubmed-article:9404420 | pubmed:meshHeading | pubmed-meshheading:9404420-... | lld:pubmed |
| pubmed-article:9404420 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9404420 | pubmed:articleTitle | [Gene transfer of interferon beta inhibits vascular smooth muscle cell proliferation in vitro and in animal model of arterial injury]. | lld:pubmed |
| pubmed-article:9404420 | pubmed:affiliation | Institut de pharmacologie, université Louis-Pasteur, Strasbourg. | lld:pubmed |
| pubmed-article:9404420 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9404420 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:9404420 | pubmed:publicationType | English Abstract | lld:pubmed |
| pubmed-article:9404420 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
