| pubmed-article:9401054 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9401054 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:9401054 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:9401054 | lifeskim:mentions | umls-concept:C0132555 | lld:lifeskim |
| pubmed-article:9401054 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:9401054 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:9401054 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:9401054 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:9401054 | pubmed:dateCreated | 1998-1-23 | lld:pubmed |
| pubmed-article:9401054 | pubmed:abstractText | Induction of nitric oxide synthase (iNOS) and production of the toxic metabolite nitric oxide (NO) is one of the interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha) regulated effector mechanisms that can lead to apoptosis of haemopoietic progenitor cells. Fas-receptor (Fas-R) expression can be stimulated by IFN-gamma and TNF-alpha. Transactivation of iNOS, and possibly Fas-R promoters, by interferon regulatory factor-1 expressed in response to IFN-gamma may be a part of the iNOS transduction pathway. We investigated whether the effects of Fas-R triggering in haemopoietic cells were mediated by NO. On Western blotting, we observed that Fas-receptor agonist, monoclonal antibody CH11. enhanced expression of iNOS. As shown by the reverse transcription polymerase chain reaction. CH11 also induced iNOS mRNA expression in purified CD34+ cells. To determine whether NO was involved in Fas-mediated apoptosis we inhibited iNOS-catalysed production of NO using anti-sense (AS) oligodeoxynucleotides (ODN) directed against iNOS mRNA. After culture of haemopoietic cells in the presence of AS-ODN, iNOS expression decreased and was no longer enhanced by Fas. This effect was associated with the prevention of Fas-mediated apoptosis, as determined by a DNA fragmentation and terminal deoxynucleotidyl transferase staining. In colony assays, specific AS-oligonucleotides prevented FAS-mediated inhibition of colony formation by total bone marrow and CD34+ progenitor cells. Our data suggest that the inhibitory effects of Fas, including induction of apoptosis, are mediated by effector mechanisms that may be similar to those described for IFN-gamma and TNF-alpha. | lld:pubmed |
| pubmed-article:9401054 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9401054 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9401054 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9401054 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9401054 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9401054 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9401054 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9401054 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9401054 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9401054 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9401054 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9401054 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:9401054 | pubmed:issn | 0007-1048 | lld:pubmed |
| pubmed-article:9401054 | pubmed:author | pubmed-author:SatoTT | lld:pubmed |
| pubmed-article:9401054 | pubmed:author | pubmed-author:RotoliBB | lld:pubmed |
| pubmed-article:9401054 | pubmed:author | pubmed-author:YoungN SNS | lld:pubmed |
| pubmed-article:9401054 | pubmed:author | pubmed-author:MaciejewskiJ... | lld:pubmed |
| pubmed-article:9401054 | pubmed:author | pubmed-author:SelleriCC | lld:pubmed |
| pubmed-article:9401054 | pubmed:author | pubmed-author:RaiolaA MAM | lld:pubmed |
| pubmed-article:9401054 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9401054 | pubmed:volume | 99 | lld:pubmed |
| pubmed-article:9401054 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9401054 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9401054 | pubmed:pagination | 481-9 | lld:pubmed |
| pubmed-article:9401054 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9401054 | pubmed:meshHeading | pubmed-meshheading:9401054-... | lld:pubmed |
| pubmed-article:9401054 | pubmed:meshHeading | pubmed-meshheading:9401054-... | lld:pubmed |
| pubmed-article:9401054 | pubmed:meshHeading | pubmed-meshheading:9401054-... | lld:pubmed |
| pubmed-article:9401054 | pubmed:meshHeading | pubmed-meshheading:9401054-... | lld:pubmed |
| pubmed-article:9401054 | pubmed:meshHeading | pubmed-meshheading:9401054-... | lld:pubmed |
| pubmed-article:9401054 | pubmed:meshHeading | pubmed-meshheading:9401054-... | lld:pubmed |
| pubmed-article:9401054 | pubmed:meshHeading | pubmed-meshheading:9401054-... | lld:pubmed |
| pubmed-article:9401054 | pubmed:meshHeading | pubmed-meshheading:9401054-... | lld:pubmed |
| pubmed-article:9401054 | pubmed:meshHeading | pubmed-meshheading:9401054-... | lld:pubmed |
| pubmed-article:9401054 | pubmed:meshHeading | pubmed-meshheading:9401054-... | lld:pubmed |
| pubmed-article:9401054 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9401054 | pubmed:articleTitle | Induction of nitric oxide synthase is involved in the mechanism of Fas-mediated apoptosis in haemopoietic cells. | lld:pubmed |
| pubmed-article:9401054 | pubmed:affiliation | Division of Haematology, Federico II University Medical School, Naples, Italy. | lld:pubmed |
| pubmed-article:9401054 | pubmed:publicationType | Journal Article | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9401054 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9401054 | lld:pubmed |
