| pubmed-article:9398165 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9398165 | lifeskim:mentions | umls-concept:C0020281 | lld:lifeskim |
| pubmed-article:9398165 | lifeskim:mentions | umls-concept:C0669368 | lld:lifeskim |
| pubmed-article:9398165 | lifeskim:mentions | umls-concept:C1522492 | lld:lifeskim |
| pubmed-article:9398165 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:9398165 | pubmed:issue | 47 | lld:pubmed |
| pubmed-article:9398165 | pubmed:dateCreated | 1998-1-8 | lld:pubmed |
| pubmed-article:9398165 | pubmed:abstractText | Neuronal nitric oxide synthase (nNOS) catalyzes the oxidation of NG-hydroxy-L-arginine (NHA) by hydrogen peroxide. The amino acid products were characterized by high-performance liquid chromatography/mass spectrometry of the o-phthalaldehyde/2-mercaptoethanol derivatives and identified as citrulline and N delta-cyanoornithine (CN-orn). The assignment of CN-orn was confirmed by independent chemical synthesis and comparison of the properties of the enzyme-derived product with those of synthetic CN-orn. The inorganic products detected in the enzymatic reaction were NO2- and NO3-, presumably from oxidation of NO-. The reaction of H2O2 and NHA with nNOS was at least 10-fold slower than the reaction of NADPH, O2, and NHA (Vmax,app = 49 +/- 2 nmol min-1 mg-1 for the reactions with 10 microM added H4B). The reaction exhibited saturation kinetics with respect to hydrogen peroxide [K(m,app)(H2O2) = 10 +/- 1 mM for the reactions with 10 microM added H4B]. No H2O2-dependent reaction was observed with L-arginine as the amino acid substrate. The different products for the NADPH- and H2O2-dependent transformations of NHA are of mechanistic significance in the NOS reaction. | lld:pubmed |
| pubmed-article:9398165 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9398165 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9398165 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9398165 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9398165 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9398165 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9398165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9398165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9398165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9398165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9398165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9398165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9398165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9398165 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9398165 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:9398165 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:9398165 | pubmed:author | pubmed-author:WishnokJ SJS | lld:pubmed |
| pubmed-article:9398165 | pubmed:author | pubmed-author:MarlettaM AMA | lld:pubmed |
| pubmed-article:9398165 | pubmed:author | pubmed-author:ClagueM JMJ | lld:pubmed |
| pubmed-article:9398165 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9398165 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:9398165 | pubmed:volume | 36 | lld:pubmed |
| pubmed-article:9398165 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9398165 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9398165 | pubmed:pagination | 14465-73 | lld:pubmed |
| pubmed-article:9398165 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:9398165 | pubmed:meshHeading | pubmed-meshheading:9398165-... | lld:pubmed |
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| pubmed-article:9398165 | pubmed:meshHeading | pubmed-meshheading:9398165-... | lld:pubmed |
| pubmed-article:9398165 | pubmed:meshHeading | pubmed-meshheading:9398165-... | lld:pubmed |
| pubmed-article:9398165 | pubmed:meshHeading | pubmed-meshheading:9398165-... | lld:pubmed |
| pubmed-article:9398165 | pubmed:meshHeading | pubmed-meshheading:9398165-... | lld:pubmed |
| pubmed-article:9398165 | pubmed:meshHeading | pubmed-meshheading:9398165-... | lld:pubmed |
| pubmed-article:9398165 | pubmed:meshHeading | pubmed-meshheading:9398165-... | lld:pubmed |
| pubmed-article:9398165 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9398165 | pubmed:articleTitle | Formation of N delta-cyanoornithine from NG-hydroxy-L-arginine and hydrogen peroxide by neuronal nitric oxide synthase: implications for mechanism. | lld:pubmed |
| pubmed-article:9398165 | pubmed:affiliation | Interdepartmental Program in Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor 48109-1065, USA. | lld:pubmed |
| pubmed-article:9398165 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9398165 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9398165 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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