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pubmed-article:9395877pubmed:abstractTextDetermination of excised cornea viability is of interest for transplant-storage evaluation, but also for in vitro diffusion-study design and ocular-toxicity assessment. By using simultaneous vital staining by calcein AM (CAM) and ethidium homodimer-1 (EH-1), as "live" and "dead" probes, respectively, we developed a confocal laser scanning microscopy (CLSM) assay to determine epithelial and endothelial viability and estimate cornea thickness.lld:pubmed
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pubmed-article:9395877pubmed:articleTitleAssessment of cornea viability by confocal laser scanning microscopy and MTT assay.lld:pubmed
pubmed-article:9395877pubmed:affiliationDepartment of Biopharmaceutical Sciences, School of Pharmacy, University of California at San Francisco 94143-0446, USA.lld:pubmed
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