| pubmed-article:9378977 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9378977 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:9378977 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:9378977 | lifeskim:mentions | umls-concept:C0205147 | lld:lifeskim |
| pubmed-article:9378977 | lifeskim:mentions | umls-concept:C0012881 | lld:lifeskim |
| pubmed-article:9378977 | lifeskim:mentions | umls-concept:C0004358 | lld:lifeskim |
| pubmed-article:9378977 | lifeskim:mentions | umls-concept:C0282056 | lld:lifeskim |
| pubmed-article:9378977 | lifeskim:mentions | umls-concept:C1510827 | lld:lifeskim |
| pubmed-article:9378977 | lifeskim:mentions | umls-concept:C1883712 | lld:lifeskim |
| pubmed-article:9378977 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:9378977 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:9378977 | pubmed:dateCreated | 1997-11-7 | lld:pubmed |
| pubmed-article:9378977 | pubmed:abstractText | The generation of terminal deoxynucleotidyl transferase knockout mice (TdT0) has demonstrated that TdT is the only major activity involved in N region addition. This enzyme generates diversity by adding random nucleotides at the V-D-J junctions and by disrupting the formation of repetitive "homology-directed" junctions. Several studies have demonstrated that the Ig heavy chain third complementarity-determining region (H-CDR3) and the N region play a critical role: 1) in distinguishing between polyreactive and monospecific combining sites in natural and Ag-induced Abs; and 2) in the specificity and polyreactivity of natural autoantibodies (autoAbs) and in particular of anti-DNA Abs. To examine the impact of the lack of TdT on the natural autoAb repertoire in adult mice, we have stimulated TdT0 and TdT+ littermates with LPS. Serum studies demonstrate that TdT is not critical for the generation of B cells expressing autoAbs including anti-DNA Abs and rheumatoid factors. However, the generation of a large collection of hybridomas indicates that the frequencies of these cells are reduced in TdT0 mice mainly due to a lower incidence of polyreactivity; also, the lack of N region diversity seems to negatively affect the affinity of anti-DNA Abs. The physiologic relevance of these data is discussed. | lld:pubmed |
| pubmed-article:9378977 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9378977 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9378977 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:9378977 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9378977 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9378977 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9378977 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9378977 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9378977 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9378977 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9378977 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9378977 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:9378977 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:9378977 | pubmed:author | pubmed-author:WellerSS | lld:pubmed |
| pubmed-article:9378977 | pubmed:author | pubmed-author:MartinTT | lld:pubmed |
| pubmed-article:9378977 | pubmed:author | pubmed-author:PasqualiJ LJL | lld:pubmed |
| pubmed-article:9378977 | pubmed:author | pubmed-author:CondeCC | lld:pubmed |
| pubmed-article:9378977 | pubmed:author | pubmed-author:KnappA MAM | lld:pubmed |
| pubmed-article:9378977 | pubmed:author | pubmed-author:LevalloisHH | lld:pubmed |
| pubmed-article:9378977 | pubmed:author | pubmed-author:GilfillanSS | lld:pubmed |
| pubmed-article:9378977 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9378977 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:9378977 | pubmed:volume | 159 | lld:pubmed |
| pubmed-article:9378977 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9378977 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9378977 | pubmed:pagination | 3890-8 | lld:pubmed |
| pubmed-article:9378977 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:meshHeading | pubmed-meshheading:9378977-... | lld:pubmed |
| pubmed-article:9378977 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9378977 | pubmed:articleTitle | Autoantibodies in mice lacking terminal deoxynucleotidyl transferase: evidence for a role of N region addition in the polyreactivity and in the affinities of anti-DNA antibodies. | lld:pubmed |
| pubmed-article:9378977 | pubmed:affiliation | Laboratory of Immunopathology, Central Hospital, University Hospitals of Strasbourg, France. | lld:pubmed |
| pubmed-article:9378977 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9378977 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:21673 | entrezgene:pubmed | pubmed-article:9378977 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9378977 | lld:pubmed |
