| pubmed-article:9376596 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9376596 | lifeskim:mentions | umls-concept:C0086427 | lld:lifeskim |
| pubmed-article:9376596 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9376596 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:9376596 | lifeskim:mentions | umls-concept:C0919490 | lld:lifeskim |
| pubmed-article:9376596 | lifeskim:mentions | umls-concept:C0521447 | lld:lifeskim |
| pubmed-article:9376596 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:9376596 | lifeskim:mentions | umls-concept:C0040648 | lld:lifeskim |
| pubmed-article:9376596 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:9376596 | lifeskim:mentions | umls-concept:C0004083 | lld:lifeskim |
| pubmed-article:9376596 | lifeskim:mentions | umls-concept:C0039371 | lld:lifeskim |
| pubmed-article:9376596 | lifeskim:mentions | umls-concept:C0253464 | lld:lifeskim |
| pubmed-article:9376596 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:9376596 | pubmed:dateCreated | 1997-11-12 | lld:pubmed |
| pubmed-article:9376596 | pubmed:abstractText | The human T-cell leukemia virus type I (HTLV-I), which infects a wide variety of mammalian cells including monocytes and macrophages, encodes a transactivating protein designated as Tax. We now report that Tax induces the human prointerleukin-1beta (IL1B) gene promoter in monocytic cells. In our transient transfection assays using human THP-1 monocytic cells, a chloramphenicol acetyltransferase (CAT) construct containing the IL1B promoter sequence between positions -131 and +12 showed an approximately 90-fold increase in activity following cotransfection of a Tax expression vector. Moreover, Tax synergized with lipopolysaccharide (LPS) to induce the IL1B promoter activity. Analyses of specific nucleotide substitutions further indicated that the Tax-induced transcriptional activation requires two transcription factor binding motifs within the IL1B promoter; one is a binding site for nuclear factor (NF)-IL6 (CCAAT/enhancer binding protein beta, C/EBP beta), which belongs to the basic region-leucine zipper (bZIP) family and the other for Spi-1 (PU.1), which is an Ets family protein found principally in monocytes, macrophages, and B lymphocytes. In electrophoretic mobility shift assays (EMSA) using in vivo THP-1 nuclear extracts, Tax expression in THP-1 monocytic cells significantly increased binding of the two factors to their target IL1B promoter sequences. However, in contrast to NF-IL6 and Spi-1, DNA binding activity of Oct-1, an ubiquitously expressed octamer-binding protein was not affected by Tax. Additional EMSA using in vitro translated proteins also showed that recombinant Tax enhances DNA binding of both of recombinant NF-IL6 and Spi-1 proteins. These data were supported by our glutathione S-transferase (GST)-pull-down data, which indicated that Tax physically interacts with the two proteins. Based on the results obtained from the present study, we conclude that the IL1B promoter is a Tax-responsive sequence as a result of ability of Tax to induce binding of NF-IL6 and Spi-1 to the IL1B promoter sequence through protein-protein interaction. | lld:pubmed |
| pubmed-article:9376596 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9376596 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9376596 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:9376596 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9376596 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:9376596 | pubmed:issn | 0006-4971 | lld:pubmed |
| pubmed-article:9376596 | pubmed:author | pubmed-author:NakanishiMM | lld:pubmed |
| pubmed-article:9376596 | pubmed:author | pubmed-author:MurakamiSS | lld:pubmed |
| pubmed-article:9376596 | pubmed:author | pubmed-author:EtoSS | lld:pubmed |
| pubmed-article:9376596 | pubmed:author | pubmed-author:OgawaRR | lld:pubmed |
| pubmed-article:9376596 | pubmed:author | pubmed-author:MorimotoII | lld:pubmed |
| pubmed-article:9376596 | pubmed:author | pubmed-author:AuronP EPE | lld:pubmed |
| pubmed-article:9376596 | pubmed:author | pubmed-author:KominatoYY | lld:pubmed |
| pubmed-article:9376596 | pubmed:author | pubmed-author:TsukadaJJ | lld:pubmed |
| pubmed-article:9376596 | pubmed:author | pubmed-author:MisagoMM | lld:pubmed |
| pubmed-article:9376596 | pubmed:author | pubmed-author:TonaiSS | lld:pubmed |
| pubmed-article:9376596 | pubmed:author | pubmed-author:SerinoYY | lld:pubmed |
| pubmed-article:9376596 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9376596 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:9376596 | pubmed:volume | 90 | lld:pubmed |
| pubmed-article:9376596 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9376596 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9376596 | pubmed:pagination | 3142-53 | lld:pubmed |
| pubmed-article:9376596 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:9376596 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9376596 | pubmed:articleTitle | Human T-cell leukemia virus type I Tax transactivates the promoter of human prointerleukin-1beta gene through association with two transcription factors, nuclear factor-interleukin-6 and Spi-1. | lld:pubmed |
| pubmed-article:9376596 | pubmed:affiliation | First Department of Internal Medicine, School of Medicine and the School of Health Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan. | lld:pubmed |
| pubmed-article:9376596 | pubmed:publicationType | Journal Article | lld:pubmed |
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