| pubmed-article:9368620 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9368620 | lifeskim:mentions | umls-concept:C0023270 | lld:lifeskim |
| pubmed-article:9368620 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9368620 | lifeskim:mentions | umls-concept:C0003315 | lld:lifeskim |
| pubmed-article:9368620 | lifeskim:mentions | umls-concept:C0085295 | lld:lifeskim |
| pubmed-article:9368620 | lifeskim:mentions | umls-concept:C0104998 | lld:lifeskim |
| pubmed-article:9368620 | lifeskim:mentions | umls-concept:C0021764 | lld:lifeskim |
| pubmed-article:9368620 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:9368620 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:9368620 | lifeskim:mentions | umls-concept:C1705431 | lld:lifeskim |
| pubmed-article:9368620 | lifeskim:mentions | umls-concept:C1819464 | lld:lifeskim |
| pubmed-article:9368620 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:9368620 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:9368620 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:9368620 | pubmed:dateCreated | 1997-12-10 | lld:pubmed |
| pubmed-article:9368620 | pubmed:abstractText | LeIF, a gene homologue of the eukaryotic initiation factor 4A was first described as a leishmanial antigen that induced a Th1-type T cell response in peripheral blood mononuclear cells (PBMC) from leishmaniasis patients. Moreover, the interferon (IFN)-gamma production by PBMC was found to be interleukin (IL)-12 dependent. Herein, we characterize the effects of LeIF on cytokine production and expression of surface molecules by normal human monocytes as well as by monocyte-derived macrophages and dendritic cells (MoDC). LeIF was a strong inducer of IL-12 and, to a lesser extent, of IL-10 and tumor necrosis factor (TNF)-alpha in macrophages and MoDC. IL-12 production did not require CD40 triggering, confirming that the ability of LeIF to induce IL-12 was not mediated through an effect on T cells. However, addition of soluble CD40 ligand (L) synergistically augmented IL-12 production in macrophages and MoDC. The cytokine-inducing activity of LeIF is located in the N-terminal portion of the molecule and was both proteinase K sensitive and polymyxin B resistant. LeIF, lipopolysaccharide and fixed Staphylococcus aureus all induced comparable amounts of IL-12, validating the potent cytokine-inducing effects of LeIF. Moreover, of these stimuli, LeIF had the highest IL-12/IL-10 and IL-12/TNF-alpha ratio demonstrating the preference of LeIF for IL-12 induction. Studies investigating the expression of surface molecules showed that LeIF up-regulated B7-1 and CD54 (ICAM-1) on macrophages and MoDC. To our knowledge this is the first report describing IL-12 production, up-regulation of co-stimulatory and intercellular adhesion molecules by monocytic antigen-presenting cells in response to a protein from a pathogenic microorganism. These immunomodulatory characteristics of LeIF might be excellent properties for a Th1-type adjuvant. | lld:pubmed |
| pubmed-article:9368620 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9368620 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9368620 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9368620 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9368620 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9368620 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9368620 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:9368620 | pubmed:issn | 0014-2980 | lld:pubmed |
| pubmed-article:9368620 | pubmed:author | pubmed-author:ProbstPP | lld:pubmed |
| pubmed-article:9368620 | pubmed:author | pubmed-author:ReedS GSG | lld:pubmed |
| pubmed-article:9368620 | pubmed:author | pubmed-author:GrabsteinK... | lld:pubmed |
| pubmed-article:9368620 | pubmed:author | pubmed-author:SkeikyY AYA | lld:pubmed |
| pubmed-article:9368620 | pubmed:author | pubmed-author:SteevesMM | lld:pubmed |
| pubmed-article:9368620 | pubmed:author | pubmed-author:GervassiAA | lld:pubmed |
| pubmed-article:9368620 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9368620 | pubmed:volume | 27 | lld:pubmed |
| pubmed-article:9368620 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9368620 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9368620 | pubmed:pagination | 2634-42 | lld:pubmed |
| pubmed-article:9368620 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:9368620 | pubmed:meshHeading | pubmed-meshheading:9368620-... | lld:pubmed |
| pubmed-article:9368620 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9368620 | pubmed:articleTitle | A Leishmania protein that modulates interleukin (IL)-12, IL-10 and tumor necrosis factor-alpha production and expression of B7-1 in human monocyte-derived antigen-presenting cells. | lld:pubmed |
| pubmed-article:9368620 | pubmed:affiliation | Infectious Disease Research Institute, Seattle, WA, USA. probst@corixa.com | lld:pubmed |
| pubmed-article:9368620 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9368620 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9368620 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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