| pubmed-article:9351889 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9351889 | lifeskim:mentions | umls-concept:C0014609 | lld:lifeskim |
| pubmed-article:9351889 | lifeskim:mentions | umls-concept:C0001455 | lld:lifeskim |
| pubmed-article:9351889 | lifeskim:mentions | umls-concept:C0012472 | lld:lifeskim |
| pubmed-article:9351889 | lifeskim:mentions | umls-concept:C1704675 | lld:lifeskim |
| pubmed-article:9351889 | lifeskim:mentions | umls-concept:C0311417 | lld:lifeskim |
| pubmed-article:9351889 | lifeskim:mentions | umls-concept:C1621850 | lld:lifeskim |
| pubmed-article:9351889 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:9351889 | pubmed:dateCreated | 1998-2-19 | lld:pubmed |
| pubmed-article:9351889 | pubmed:abstractText | Previous work from this laboratory has shown that apical membrane sodium channel activity is stimulated by serosal hyposmotic solutions (Wills, Millinoff & Crowe, 1991). In the present study, we determined whether this stimulation of sodium transport is additive with the actions of prostaglandin E2 (PGE2) or cyclic AMP (cAMP). Addition of exogenous PGE2 (100 nM; serosal bath) to isosmotic solutions led to large increases in the amiloride-sensitive short-circuit current (Isc) and transepithelial conductance (Gt), whereas no significant effects of PGE2 were observed in hyposmotic serosal solutions. Subsequent addition of mucosal amiloride reduced Isc by approximately 95% and Gt by approximately 60%. Inhibition of endogenous PGE2 production by blockers of phospholipase A2 activity (quinacrine or 3[4-octadecyl]-benzoylacrylic acid; OBBA), or inhibition of cyclooxygenase activity by indomethacin reduced the stimulation of Isc and Gt by hyposmotic solutions. Addition of forskolin (FSK) or 3-Isobutyl-1-methylxanthine (IBMX) also resulted in approximately twofold increases in the amiloride-sensitive Isc and Gt and abolished the effects of subsequent hyposmotic challenge. The effects of forskolin, PGE2, and hyposmotic challenge were diminished by pretreatment with H89, a protein kinase A (PKA) inhibitor. We conclude that osmotic regulation of sodium channel activity interacts with multiple intracellular signaling pathways, specifically the arachidonic acid metabolic pathway and the cAMP/PKA intracellular messenger cascade. | lld:pubmed |
| pubmed-article:9351889 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9351889 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9351889 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9351889 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9351889 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9351889 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9351889 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9351889 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9351889 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9351889 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9351889 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9351889 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9351889 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:9351889 | pubmed:issn | 0022-2631 | lld:pubmed |
| pubmed-article:9351889 | pubmed:author | pubmed-author:WillsN KNK | lld:pubmed |
| pubmed-article:9351889 | pubmed:author | pubmed-author:OHWW | lld:pubmed |
| pubmed-article:9351889 | pubmed:author | pubmed-author:MatsumotoP... | lld:pubmed |
| pubmed-article:9351889 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9351889 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:9351889 | pubmed:volume | 160 | lld:pubmed |
| pubmed-article:9351889 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9351889 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9351889 | pubmed:pagination | 27-38 | lld:pubmed |
| pubmed-article:9351889 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:9351889 | pubmed:meshHeading | pubmed-meshheading:9351889-... | lld:pubmed |
| pubmed-article:9351889 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9351889 | pubmed:articleTitle | Osmotic regulation of Na+ transport across A6 epithelium: interactions with prostaglandin E2 and cyclic AMP. | lld:pubmed |
| pubmed-article:9351889 | pubmed:affiliation | Department of Physiology & Biophysics, University of Texas Medical Branch, Galveston 77555, USA. | lld:pubmed |
| pubmed-article:9351889 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9351889 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9351889 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9351889 | lld:pubmed |
