| pubmed-article:9328803 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9328803 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:9328803 | lifeskim:mentions | umls-concept:C0026844 | lld:lifeskim |
| pubmed-article:9328803 | lifeskim:mentions | umls-concept:C1135918 | lld:lifeskim |
| pubmed-article:9328803 | lifeskim:mentions | umls-concept:C0035096 | lld:lifeskim |
| pubmed-article:9328803 | lifeskim:mentions | umls-concept:C0205276 | lld:lifeskim |
| pubmed-article:9328803 | lifeskim:mentions | umls-concept:C0032200 | lld:lifeskim |
| pubmed-article:9328803 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:9328803 | lifeskim:mentions | umls-concept:C1514485 | lld:lifeskim |
| pubmed-article:9328803 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:9328803 | pubmed:dateCreated | 1997-11-26 | lld:pubmed |
| pubmed-article:9328803 | pubmed:abstractText | This experiment was designed to investigate the possible involvement of angiotensin II (Ang II) and platelet-derived growth factor (PDGF) in the mechanism underlying stretch-induced proliferation of vascular smooth muscle cells (SMCs). SMCs from the rabbit aortic media were grown on polystyrene rubber-bottomed dishes coated with type I collagen. Cells were stretched cyclically by a vacuum-operated downward flexion of the culture dish bottom. A 1.4- to 1.6-fold increase in proliferation of SMCs was induced by cyclic stretching, as determined by [3H]-thymidine incorporation, in a stretch force-dependent manner in the range of 5% to 15% elongation, 30 cycles/min for 24 h. Expression of PDGF-B chain mRNA was up-regulated in a time-dependent manner in the range of 2 to 24 h, 10% elongation, and 30 cycles/min. Saralasin, a selective antagonist of Ang II, and captopril, an angiotensin I converting enzyme inhibitor, significantly suppressed the stretch-induced proliferation of SMCs. Blockade of angiotensinogen mRNA translation by antisense oligonucleotide inhibited proliferation under the mechanical strain. Stretch-induced proliferation was inhibited by 78% in the presence of anti-PDGF-AB neutralizing antibody. Increased expression of PDGF-B chain mRNA under the mechanical strain was inhibited by treatment with saralasin. Our results indicate that the stretch-induced proliferation of cultured SMCs is mediated at least in part via increased production of Ang II by the local renin-angiotensin system and a subsequent up-regulation of PDGF-B chain mRNA in an autocrine-paracrine manner. | lld:pubmed |
| pubmed-article:9328803 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9328803 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9328803 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9328803 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9328803 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9328803 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9328803 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9328803 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9328803 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9328803 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9328803 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9328803 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9328803 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:9328803 | pubmed:issn | 0916-9636 | lld:pubmed |
| pubmed-article:9328803 | pubmed:author | pubmed-author:OoshimaAA | lld:pubmed |
| pubmed-article:9328803 | pubmed:author | pubmed-author:UenoHH | lld:pubmed |
| pubmed-article:9328803 | pubmed:author | pubmed-author:LUEE | lld:pubmed |
| pubmed-article:9328803 | pubmed:author | pubmed-author:MuragakiYY | lld:pubmed |
| pubmed-article:9328803 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9328803 | pubmed:volume | 20 | lld:pubmed |
| pubmed-article:9328803 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9328803 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9328803 | pubmed:pagination | 217-23 | lld:pubmed |
| pubmed-article:9328803 | pubmed:dateRevised | 2003-11-14 | lld:pubmed |
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| pubmed-article:9328803 | pubmed:meshHeading | pubmed-meshheading:9328803-... | lld:pubmed |
| pubmed-article:9328803 | pubmed:meshHeading | pubmed-meshheading:9328803-... | lld:pubmed |
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| pubmed-article:9328803 | pubmed:meshHeading | pubmed-meshheading:9328803-... | lld:pubmed |
| pubmed-article:9328803 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9328803 | pubmed:articleTitle | Stretch-induced proliferation of cultured vascular smooth muscle cells and a possible involvement of local renin-angiotensin system and platelet-derived growth factor (PDGF). | lld:pubmed |
| pubmed-article:9328803 | pubmed:affiliation | Department of Pathology, Wakayama Medical College, Japan. | lld:pubmed |
| pubmed-article:9328803 | pubmed:publicationType | Journal Article | lld:pubmed |
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