| pubmed-article:9324303 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9324303 | lifeskim:mentions | umls-concept:C0027651 | lld:lifeskim |
| pubmed-article:9324303 | lifeskim:mentions | umls-concept:C0004561 | lld:lifeskim |
| pubmed-article:9324303 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:9324303 | lifeskim:mentions | umls-concept:C0021027 | lld:lifeskim |
| pubmed-article:9324303 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:9324303 | lifeskim:mentions | umls-concept:C0679199 | lld:lifeskim |
| pubmed-article:9324303 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:9324303 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:9324303 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:9324303 | pubmed:dateCreated | 1997-10-23 | lld:pubmed |
| pubmed-article:9324303 | pubmed:abstractText | Several methods have been developed for the detection of minimal residual disease (MRD) in B cell tumors. Chromosomal translocations or the rearrangement of the immunoglobulin heavy chain (IgH) and T cell receptor genes are generally employed. We report a novel PCR method to detect MRD using IgH genes. IgH rearranged variable region (VDJ) were amplified from tumor specimens using consensus primers for variable and joining region genes. Complementarity-determining regions (CDR) were identified and used to generate tumor-specific primers. Two-round amplifications using primers derived from CDRs and joining or constant regions were performed for MRD detection. IgH nested-PCR approach was tested on a panel of 75 B cell tumors including acute lymphoblastic and chronic lymphocytic leukemias, non-Hodgkin's lymphomas and multiple myelomas. A VDJ sequence was obtained in 62 out of 75 cases (83%). Sensitivity using DNA or cDNA templates was 10(-5) and (-6), respectively. This method is specific and sensitive and provides a simple, non-radioactive approach for the evaluation of MRD in B cell tumors. | lld:pubmed |
| pubmed-article:9324303 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9324303 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9324303 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9324303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9324303 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9324303 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9324303 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:9324303 | pubmed:issn | 0887-6924 | lld:pubmed |
| pubmed-article:9324303 | pubmed:author | pubmed-author:PileriAA | lld:pubmed |
| pubmed-article:9324303 | pubmed:author | pubmed-author:BoccadoroMM | lld:pubmed |
| pubmed-article:9324303 | pubmed:author | pubmed-author:CorradiniPP | lld:pubmed |
| pubmed-article:9324303 | pubmed:author | pubmed-author:AstolfiMM | lld:pubmed |
| pubmed-article:9324303 | pubmed:author | pubmed-author:GribbenJ GJG | lld:pubmed |
| pubmed-article:9324303 | pubmed:author | pubmed-author:LadettoMM | lld:pubmed |
| pubmed-article:9324303 | pubmed:author | pubmed-author:VoenaCC | lld:pubmed |
| pubmed-article:9324303 | pubmed:author | pubmed-author:ProvanDD | lld:pubmed |
| pubmed-article:9324303 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9324303 | pubmed:volume | 11 | lld:pubmed |
| pubmed-article:9324303 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9324303 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9324303 | pubmed:pagination | 1793-8 | lld:pubmed |
| pubmed-article:9324303 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:9324303 | pubmed:meshHeading | pubmed-meshheading:9324303-... | lld:pubmed |
| pubmed-article:9324303 | pubmed:meshHeading | pubmed-meshheading:9324303-... | lld:pubmed |
| pubmed-article:9324303 | pubmed:meshHeading | pubmed-meshheading:9324303-... | lld:pubmed |
| pubmed-article:9324303 | pubmed:meshHeading | pubmed-meshheading:9324303-... | lld:pubmed |
| pubmed-article:9324303 | pubmed:meshHeading | pubmed-meshheading:9324303-... | lld:pubmed |
| pubmed-article:9324303 | pubmed:meshHeading | pubmed-meshheading:9324303-... | lld:pubmed |
| pubmed-article:9324303 | pubmed:meshHeading | pubmed-meshheading:9324303-... | lld:pubmed |
| pubmed-article:9324303 | pubmed:meshHeading | pubmed-meshheading:9324303-... | lld:pubmed |
| pubmed-article:9324303 | pubmed:meshHeading | pubmed-meshheading:9324303-... | lld:pubmed |
| pubmed-article:9324303 | pubmed:meshHeading | pubmed-meshheading:9324303-... | lld:pubmed |
| pubmed-article:9324303 | pubmed:meshHeading | pubmed-meshheading:9324303-... | lld:pubmed |
| pubmed-article:9324303 | pubmed:meshHeading | pubmed-meshheading:9324303-... | lld:pubmed |
| pubmed-article:9324303 | pubmed:meshHeading | pubmed-meshheading:9324303-... | lld:pubmed |
| pubmed-article:9324303 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9324303 | pubmed:articleTitle | A novel nested-PCR strategy for the detection of rearranged immunoglobulin heavy-chain genes in B cell tumors. | lld:pubmed |
| pubmed-article:9324303 | pubmed:affiliation | Dipartimento di Medicina ed Oncologia Sperimentale, Azienda Ospedaliera San Giovanni Battista, Torino, Italy. | lld:pubmed |
| pubmed-article:9324303 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:9324303 | pubmed:publicationType | Technical Report | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9324303 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9324303 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9324303 | lld:pubmed |
