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pubmed-article:9290067pubmed:abstractTextAn obligately methylotrophic organism was isolated from a water well that manifested symptoms of biofouling. The isolate was appendaged and utilized methylamine, dimethylamine, trimethylamine, or methanol as the sole carbon and energy source. The isolate exhibited hydroxypyruvate reductase activity, suggesting C1-assimilation via the serine pathway. Fatty acid profiling indicated the predominance of 18:1 cis-fatty acids. The isolate did not grow anaerobically with nitrate as the final electron acceptor. Genomic DNA from the isolate did not hybridize against the narG gene, which encodes the alpha subunit of dissimilatory nitrate reductase in Escherichia coli. The phenotypic data suggested the assignment of the isolate to the genus Hyphomicrobium. The identification was supported by phylogenetic characterization based on 16S rRNA sequence comparisons of the isolate.lld:pubmed
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pubmed-article:9290067pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:9290067pubmed:articleTitlePhylogenetic and narG analysis of a Hyphomicrobium isolate.lld:pubmed
pubmed-article:9290067pubmed:affiliationDepartment of Microbiology, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210-1292, USA.lld:pubmed
pubmed-article:9290067pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:9290067pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
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