| pubmed-article:9285714 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9285714 | lifeskim:mentions | umls-concept:C0036025 | lld:lifeskim |
| pubmed-article:9285714 | lifeskim:mentions | umls-concept:C0011923 | lld:lifeskim |
| pubmed-article:9285714 | lifeskim:mentions | umls-concept:C0120285 | lld:lifeskim |
| pubmed-article:9285714 | lifeskim:mentions | umls-concept:C0162768 | lld:lifeskim |
| pubmed-article:9285714 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:9285714 | pubmed:dateCreated | 1998-2-3 | lld:pubmed |
| pubmed-article:9285714 | pubmed:abstractText | Tagging expressed proteins with the green fluorescent protein (GFP) from Aequorea victoria [1] is a highly specific and sensitive technique for studying the intracellular dynamics of proteins and organelles. We have developed, as a probe, a fusion protein of the carboxyl terminus of dynein and GFP (dynein-GFP), which fluorescently labels the astral microtubules of the budding yeast Saccharomyces cerevisiae. This paper describes the modifications to our multimode microscope imaging system [2,3], the acquisition of three-dimensional (3-D) data sets and the computer processing methods we have developed to obtain time-lapse recordings of fluorescent astral microtubule dynamics and nuclear movements over the complete duration of the 90-120 minute yeast cell cycle. This required low excitation light intensity to prevent GFP photobleaching and phototoxicity, efficient light collection by the microscope optics, a cooled charge-coupled device (CCD) camera with high quantum efficiency, and image reconstruction from serial optical sections through the 6 micron-wide yeast cell to see most or all of the astral molecules. Methods are also described for combining fluorescent images of the microtubules labeled with dynein-GFP with high resolution differential interference contrast (DIC) images of nuclear and cellular morphology [4], and fluorescent images of the chromosomes stained with 4,6-diamidino-2-phenylindole (DAPI) [5]. | lld:pubmed |
| pubmed-article:9285714 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9285714 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9285714 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9285714 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9285714 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9285714 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9285714 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9285714 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:9285714 | pubmed:issn | 0960-9822 | lld:pubmed |
| pubmed-article:9285714 | pubmed:author | pubmed-author:BloomKK | lld:pubmed |
| pubmed-article:9285714 | pubmed:author | pubmed-author:SalmonE DED | lld:pubmed |
| pubmed-article:9285714 | pubmed:author | pubmed-author:YehEE | lld:pubmed |
| pubmed-article:9285714 | pubmed:author | pubmed-author:ShawS LSL | lld:pubmed |
| pubmed-article:9285714 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9285714 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:9285714 | pubmed:volume | 7 | lld:pubmed |
| pubmed-article:9285714 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9285714 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9285714 | pubmed:pagination | 701-4 | lld:pubmed |
| pubmed-article:9285714 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:9285714 | pubmed:meshHeading | pubmed-meshheading:9285714-... | lld:pubmed |
| pubmed-article:9285714 | pubmed:meshHeading | pubmed-meshheading:9285714-... | lld:pubmed |
| pubmed-article:9285714 | pubmed:meshHeading | pubmed-meshheading:9285714-... | lld:pubmed |
| pubmed-article:9285714 | pubmed:meshHeading | pubmed-meshheading:9285714-... | lld:pubmed |
| pubmed-article:9285714 | pubmed:meshHeading | pubmed-meshheading:9285714-... | lld:pubmed |
| pubmed-article:9285714 | pubmed:meshHeading | pubmed-meshheading:9285714-... | lld:pubmed |
| pubmed-article:9285714 | pubmed:meshHeading | pubmed-meshheading:9285714-... | lld:pubmed |
| pubmed-article:9285714 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9285714 | pubmed:articleTitle | Imaging green fluorescent protein fusion proteins in Saccharomyces cerevisiae. | lld:pubmed |
| pubmed-article:9285714 | pubmed:affiliation | Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3280, USA. | lld:pubmed |
| pubmed-article:9285714 | pubmed:publicationType | Journal Article | lld:pubmed |
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