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pubmed-article:9282785pubmed:abstractTextThe antigenicity and RNA genome structures of five Simbu serogroup bunyaviruses isolated in Japan and Australia were analyzed using monoclonal antibodies (Mabs) raised to Akabane (AKA) virus and oligonucleotide fingerprinting. The virion surface glycoprotein (G1) and the nucleocapsid (N) protein of heterologous viruses showed no reactivity to the Mabs, while the AKA-derived anti-G1 Mab (2F1) reacted with Peaton virus and all three AKA anti-N Mabs reacted with Tinaroo (TIN) virus at almost the same antibody titers as the homologous virus. Oligonucleotide fingerprinting analyses indicated that the three RNA species of all the viruses were unique and distinguishable. However, AKA and TIN viruses exhibited very similar S RNA oligonucleotide fingerprints, while the L and M RNA fingerprints were quite different. The S RNA sequence of TIN virus has been determined and compared with that of AKA and Aino viruses. The results revealed 95.1% S sequence homology between the AKA and TIN viruses. The antigenic and genetic comparisons of AKA and TIN viruses suggest that the two viruses may represent naturally occurring reassortant viruses.lld:pubmed
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pubmed-article:9282785pubmed:pagination205-13lld:pubmed
pubmed-article:9282785pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:9282785pubmed:articleTitleAntigenic and genetic comparisons of Japanese and Australian Simbu serogroup viruses: evidence for the recovery of natural virus reassortants.lld:pubmed
pubmed-article:9282785pubmed:affiliationNational Institute of Animal Health, Ibaraki, Japan. hmitsu@niah.affrc.go.jplld:pubmed
pubmed-article:9282785pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:9282785pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:9282785pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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