| pubmed-article:9268057 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9268057 | lifeskim:mentions | umls-concept:C1257857 | lld:lifeskim |
| pubmed-article:9268057 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:9268057 | lifeskim:mentions | umls-concept:C0031670 | lld:lifeskim |
| pubmed-article:9268057 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:9268057 | lifeskim:mentions | umls-concept:C0376315 | lld:lifeskim |
| pubmed-article:9268057 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:9268057 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:9268057 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:9268057 | pubmed:dateCreated | 1997-9-2 | lld:pubmed |
| pubmed-article:9268057 | pubmed:abstractText | Glycosylphosphatidylinositol (GPI)-specific phospholipase D (GPI-PLD) is a secretory protein present in high amounts in mammalian body fluids. Its cDNA has been isolated and encodes a signal peptide of 23 amino acids and the mature protein of 816 amino acids. We generated cDNAs encoding a signal peptide-deficient and a GPI-anchored form of GPI-PLD and transiently transfected these constructs into COS-1 cells. The signal peptide-deficient form of GPI-PLD was expressed as a 90-kDa protein that was catalytically active and was localized intracellularly. Cells transfected with cDNA encoding the GPI-anchored form of GPI-PLD expressed a catalytically active enzyme of 100 kDa that could be labelled with [3H]ethanolamine demonstrating its modification by a GPI structure. Expression of the GPI-anchored form of GPI-PLD resulted in the release of endogenous GPI-anchored alkaline phosphatase from COS-1 cells, whereas expression of the intracellular form of GPI-PLD had no effect on membrane attachment of endogenous alkaline phosphatase. Similarly, in cells cotransfected with GPI-anchored placental alkaline phosphatase (PLAP) and the GPI-anchored form of GPI-PLD, PLAP was released into the cell culture supernatant while expression of the signal peptide-deficient form of GPI-PLD did not affect the amount of cell-associated PLAP. | lld:pubmed |
| pubmed-article:9268057 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9268057 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9268057 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9268057 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9268057 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9268057 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9268057 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9268057 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9268057 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:9268057 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:9268057 | pubmed:author | pubmed-author:PangMM | lld:pubmed |
| pubmed-article:9268057 | pubmed:author | pubmed-author:BrodbeckUU | lld:pubmed |
| pubmed-article:9268057 | pubmed:author | pubmed-author:BütikoferPP | lld:pubmed |
| pubmed-article:9268057 | pubmed:author | pubmed-author:StadelmannBB | lld:pubmed |
| pubmed-article:9268057 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9268057 | pubmed:day | 24 | lld:pubmed |
| pubmed-article:9268057 | pubmed:volume | 1357 | lld:pubmed |
| pubmed-article:9268057 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9268057 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9268057 | pubmed:pagination | 329-38 | lld:pubmed |
| pubmed-article:9268057 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:9268057 | pubmed:meshHeading | pubmed-meshheading:9268057-... | lld:pubmed |
| pubmed-article:9268057 | pubmed:meshHeading | pubmed-meshheading:9268057-... | lld:pubmed |
| pubmed-article:9268057 | pubmed:meshHeading | pubmed-meshheading:9268057-... | lld:pubmed |
| pubmed-article:9268057 | pubmed:meshHeading | pubmed-meshheading:9268057-... | lld:pubmed |
| pubmed-article:9268057 | pubmed:meshHeading | pubmed-meshheading:9268057-... | lld:pubmed |
| pubmed-article:9268057 | pubmed:meshHeading | pubmed-meshheading:9268057-... | lld:pubmed |
| pubmed-article:9268057 | pubmed:meshHeading | pubmed-meshheading:9268057-... | lld:pubmed |
| pubmed-article:9268057 | pubmed:meshHeading | pubmed-meshheading:9268057-... | lld:pubmed |
| pubmed-article:9268057 | pubmed:meshHeading | pubmed-meshheading:9268057-... | lld:pubmed |
| pubmed-article:9268057 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9268057 | pubmed:articleTitle | Expression of intracellular and GPI-anchored forms of GPI-specific phospholipase D in COS-1 cells. | lld:pubmed |
| pubmed-article:9268057 | pubmed:affiliation | Institute of Biochemistry and Molecular Biology, University of Bern, Switzerland. | lld:pubmed |
| pubmed-article:9268057 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9268057 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9268057 | lld:pubmed |
