Linked Life Data

9267002

Source:http://linkedlifedata.com/resource/pubmed/id/9267002

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pubmed-article:9267002pubmed:abstractTextGenes encoding glycoprotein gH and gL homologues were localized in the genome of the gamma-herpesvirus bovine herpesvirus-4 (BHV-4). Both genes were sequenced and glutathione S-transferase fusion proteins were produced and used to immunize rabbits against the translation products of the two genes. The anti-gH serum recognized a protein with an apparent molecular mass (MM) of 110 kDa both in infected cells and in virions. This protein was sensitive to endo-beta-N-acetylglucosaminase-H (endoH) and endoglycosidase F-N-glycosidase F (endoF-PNGaseF) digestion. A protein with the same relative mobility was immunoprecipitated from infected cells radiolabelled with [3H]glucosamine which confirmed that this product (gp110), now designated BHV-4 gH, was glycosylated. Western blotting with the anti-gL serum detected in infected cells a product with an apparent MM ranging from 31-35 kDa and diffusely migrating protein species ranging from 45-65 kDa. Tunicamycin, monensin, endoH or endoF-PNGaseF treatments showed that both the 31-35 kDa and the 45-65 kDa proteins were glycosylated, gp31-35 being a precursor of the 45-65 kDa glycoprotein species. In radioimmunoprecipitation assays, the anti-gL serum immunoprecipitated from infected cells two glycosylated proteins with apparent MMs of 31-35 kDa (gp31-35) and 45-55 kDa (gp45-55). However a third glycoprotein, gp110, was also immunoprecipitated together with gp31-35 and gp45-55. gp110 and gp45-55 were subsequently confirmed to be virion glycoproteins corresponding to mature forms of BHV-4 gH and gL respectively. In addition, the present study clearly demonstrated complex formation between BHV-4 gH and gL both in virions and in infected cells.lld:pubmed
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pubmed-article:9267002pubmed:authorpubmed-author:LomontePPlld:pubmed
pubmed-article:9267002pubmed:authorpubmed-author:LyakuJ RJRlld:pubmed
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pubmed-article:9267002pubmed:volume78 ( Pt 8)lld:pubmed
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pubmed-article:9267002pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:9267002pubmed:year1997lld:pubmed
pubmed-article:9267002pubmed:articleTitleAnalysis of the biochemical properties of, and complex formation between, glycoproteins H and L of the gamma2 herpesvirus bovine herpesvirus-4.lld:pubmed
pubmed-article:9267002pubmed:affiliationLaboratory of Virology-Immunology, Faculty of Veterinary Medicine, University of Liège, Belgium. p.lomonte@vir.gla.ac.uklld:pubmed
pubmed-article:9267002pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:9267002pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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