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pubmed-article:9266176pubmed:abstractTextRecent advances in protein engineering have facilitated the development of alternative approaches to determine helix packing in polytopic membrane proteins. Using the lac permease as a paradigm, several site-directed biophysical and biochemical techniques are described which should be generally applicable.lld:pubmed
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pubmed-article:9266176pubmed:articleTitleHelix packing in polytopic membrane proteins: the lactose permease of Escherichia coli.lld:pubmed
pubmed-article:9266176pubmed:affiliationHoward Hughes Medical Institute, Department of Physiology, University of California, Los Angeles 90024-1662, USA. RonaldK@HHMI.UCLA.edulld:pubmed
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