| pubmed-article:9262372 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9262372 | lifeskim:mentions | umls-concept:C0010763 | lld:lifeskim |
| pubmed-article:9262372 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
| pubmed-article:9262372 | lifeskim:mentions | umls-concept:C0521457 | lld:lifeskim |
| pubmed-article:9262372 | lifeskim:mentions | umls-concept:C0919426 | lld:lifeskim |
| pubmed-article:9262372 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:9262372 | lifeskim:mentions | umls-concept:C0442375 | lld:lifeskim |
| pubmed-article:9262372 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:9262372 | pubmed:dateCreated | 1997-9-11 | lld:pubmed |
| pubmed-article:9262372 | pubmed:abstractText | We examined the potential for the widely consumed xenobiotic ethanol to transplacentally induce fetal rat CYP2E1. Throughout gestation, rat dams were fed a liquid diet containing 5% ethanol or two separate control diets. At 2 days before term, the dams were killed, and maternal and embryonic tissues were collected. Immunoblot analysis of microsomes from fetal liver, placenta and maternal brain revealed a band that comigrated with adult liver CYP2E1. The identity of the immunoreactive protein in placenta, brain and fetal liver was substantiated as CYP2E1 through restriction enzyme digestion of a reverse transcription-polymerase chain reaction product. Quantification of immunoblots containing microsomes from maternal and fetal liver of ethanol-treated dams displayed a 1.4- and 2.4-fold increase in CYP2E1, respectively, compared with microsomes from pair-fed controls. Chlorzoxazone and low substrate concentrations of N-nitrosodimethylamine were used as metabolic probes for CYP2E1. The rate of chlorzoxazone metabolism by maternal hepatic microsomes from dams fed the 5% ethanol diet was 2.6-fold greater than that of controls. Conversely, a negligible increase was observed in the rate of metabolism by hepatic microsomes from ethanol-exposed fetuses compared with pair-fed animals. When N-nitrosodimethylamine demethylation was examined, these same fetal samples exhibited greater rates of activity (1.5-fold) compared with microsomes from control animals. However, this increase was not as great as expected considering the 2.4-fold increase in CYP2E1 protein. Collectively, fetuses exposed to a 5% ethanol diet throughout gestation exhibited transplacental induction of an hepatic CYP2E1 that may possess different catalytic properties from the analogous adult enzyme. | lld:pubmed |
| pubmed-article:9262372 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9262372 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9262372 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9262372 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9262372 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9262372 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9262372 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9262372 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9262372 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9262372 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9262372 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9262372 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9262372 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:9262372 | pubmed:issn | 0022-3565 | lld:pubmed |
| pubmed-article:9262372 | pubmed:author | pubmed-author:SavageD DDD | lld:pubmed |
| pubmed-article:9262372 | pubmed:author | pubmed-author:SchultzE DED | lld:pubmed |
| pubmed-article:9262372 | pubmed:author | pubmed-author:RaucyJ LJL | lld:pubmed |
| pubmed-article:9262372 | pubmed:author | pubmed-author:CarpenterS... | lld:pubmed |
| pubmed-article:9262372 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9262372 | pubmed:volume | 282 | lld:pubmed |
| pubmed-article:9262372 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9262372 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9262372 | pubmed:pagination | 1028-36 | lld:pubmed |
| pubmed-article:9262372 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:9262372 | pubmed:meshHeading | pubmed-meshheading:9262372-... | lld:pubmed |
| pubmed-article:9262372 | pubmed:meshHeading | pubmed-meshheading:9262372-... | lld:pubmed |
| pubmed-article:9262372 | pubmed:meshHeading | pubmed-meshheading:9262372-... | lld:pubmed |
| pubmed-article:9262372 | pubmed:meshHeading | pubmed-meshheading:9262372-... | lld:pubmed |
| pubmed-article:9262372 | pubmed:meshHeading | pubmed-meshheading:9262372-... | lld:pubmed |
| pubmed-article:9262372 | pubmed:meshHeading | pubmed-meshheading:9262372-... | lld:pubmed |
| pubmed-article:9262372 | pubmed:meshHeading | pubmed-meshheading:9262372-... | lld:pubmed |
| pubmed-article:9262372 | pubmed:meshHeading | pubmed-meshheading:9262372-... | lld:pubmed |
| pubmed-article:9262372 | pubmed:meshHeading | pubmed-meshheading:9262372-... | lld:pubmed |
| pubmed-article:9262372 | pubmed:meshHeading | pubmed-meshheading:9262372-... | lld:pubmed |
| pubmed-article:9262372 | pubmed:meshHeading | pubmed-meshheading:9262372-... | lld:pubmed |
| pubmed-article:9262372 | pubmed:meshHeading | pubmed-meshheading:9262372-... | lld:pubmed |
| pubmed-article:9262372 | pubmed:meshHeading | pubmed-meshheading:9262372-... | lld:pubmed |
| pubmed-article:9262372 | pubmed:meshHeading | pubmed-meshheading:9262372-... | lld:pubmed |
| pubmed-article:9262372 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9262372 | pubmed:articleTitle | Ethanol-mediated transplacental induction of CYP2E1 in fetal rat liver. | lld:pubmed |
| pubmed-article:9262372 | pubmed:affiliation | University of New Mexico, College of Pharmacy, Albuquerque, USA. scarpen@agi.org | lld:pubmed |
| pubmed-article:9262372 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9262372 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9262372 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9262372 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9262372 | lld:pubmed |
