| pubmed-article:9255792 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9255792 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:9255792 | lifeskim:mentions | umls-concept:C0243192 | lld:lifeskim |
| pubmed-article:9255792 | lifeskim:mentions | umls-concept:C0034792 | lld:lifeskim |
| pubmed-article:9255792 | lifeskim:mentions | umls-concept:C1883036 | lld:lifeskim |
| pubmed-article:9255792 | lifeskim:mentions | umls-concept:C1260691 | lld:lifeskim |
| pubmed-article:9255792 | lifeskim:mentions | umls-concept:C0439851 | lld:lifeskim |
| pubmed-article:9255792 | lifeskim:mentions | umls-concept:C1325936 | lld:lifeskim |
| pubmed-article:9255792 | lifeskim:mentions | umls-concept:C1552596 | lld:lifeskim |
| pubmed-article:9255792 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:9255792 | lifeskim:mentions | umls-concept:C1947931 | lld:lifeskim |
| pubmed-article:9255792 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:9255792 | pubmed:dateCreated | 1997-10-14 | lld:pubmed |
| pubmed-article:9255792 | pubmed:abstractText | MuSK is a tyrosine kinase localized to the postsynaptic surface of the neuromuscular junction. We have searched for modulators of MuSK function using a library of human single chain variable region antibodies (scFv) that can be displayed on M13 phage or expressed as soluble protein. A panel of 21 independent MuSK-specific scFv, identified in a screen for binding to MuSK-Fc immunoadhesin, were examined for ability to induce proliferation in a factor dependent cell line (Ba/F3) through a chimeric receptor, MuSK-Mpl. Four of the scFv induced a proliferative response, suggesting an ability to induce dimerization of MuSK. These scFv were also able to induce tyrosine phosphorylation of full-length MuSK and retained this ability when re-engineered to be expressed as authentic (and dimeric) human IgG molecules. Addition of agonist scFv to a cultured myotube cell line induced AChR clustering and tyrosine phosphorylation. These results provide direct evidence that MuSK activation is capable of triggering a key event in neuromuscular junction formation and further demonstrate that large libraries of phage-displayed scFv provide a robust method for generating highly specific agonist agents. | lld:pubmed |
| pubmed-article:9255792 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9255792 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9255792 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9255792 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9255792 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9255792 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9255792 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9255792 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:9255792 | pubmed:issn | 1087-0156 | lld:pubmed |
| pubmed-article:9255792 | pubmed:author | pubmed-author:AdamsCC | lld:pubmed |
| pubmed-article:9255792 | pubmed:author | pubmed-author:YuanJJ | lld:pubmed |
| pubmed-article:9255792 | pubmed:author | pubmed-author:XieM HMH | lld:pubmed |
| pubmed-article:9255792 | pubmed:author | pubmed-author:GurneyAA | lld:pubmed |
| pubmed-article:9255792 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9255792 | pubmed:volume | 15 | lld:pubmed |
| pubmed-article:9255792 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9255792 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9255792 | pubmed:pagination | 768-71 | lld:pubmed |
| pubmed-article:9255792 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:9255792 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9255792 | pubmed:articleTitle | Direct demonstration of MuSK involvement in acetylcholine receptor clustering through identification of agonist ScFv. | lld:pubmed |
| pubmed-article:9255792 | pubmed:affiliation | Department of Molecular Biology, Genentech, Inc., San Francisco, CA 94080, USA. | lld:pubmed |
| pubmed-article:9255792 | pubmed:publicationType | Journal Article | lld:pubmed |
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