| pubmed-article:9241233 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9241233 | lifeskim:mentions | umls-concept:C0205419 | lld:lifeskim |
| pubmed-article:9241233 | lifeskim:mentions | umls-concept:C0205217 | lld:lifeskim |
| pubmed-article:9241233 | lifeskim:mentions | umls-concept:C1335439 | lld:lifeskim |
| pubmed-article:9241233 | lifeskim:mentions | umls-concept:C0680011 | lld:lifeskim |
| pubmed-article:9241233 | lifeskim:mentions | umls-concept:C0121925 | lld:lifeskim |
| pubmed-article:9241233 | pubmed:issue | 16 | lld:pubmed |
| pubmed-article:9241233 | pubmed:dateCreated | 1997-9-17 | lld:pubmed |
| pubmed-article:9241233 | pubmed:abstractText | Human immunodeficiency virus type 1 (HIV-1) variants with resistance mutations in the reverse transcriptase (RT) gene appear during drug therapy with the nucleoside analogue 2',3'-dideoxy-3'-thiacytidine (3TC). These 3TC-resistant RT variants have a single point mutation that changes the 184Met residue into either Val or Ile. Both codon 184 variants are frequently observed in 3TC-treated patients and can also be selected in cell culture infections. We demonstrated previously that the 184Ile and 184Val RT enzymes exhibit a processivity defect in in vitro assays, with 184Ile being the least processive enzyme [Met(wt) >Val >Ile]. In this study, we measured the polymerase fidelity of the wild-type (184Met) and 3TC-resistant RT enzymes (184Ile and 184Val) on DNA and RNA templates. Both virion- extracted and Escherichia coli -expressed recombinant RT enzymes were used to measure the nucleotide misinsertion and mispair extension efficiencies. The 3TC-resistant enzymes were more accurate than the wild-type RT protein in both type of assays. The order of accuracy observed for the codon 184 variants [Ile >Val >Met(wt)] may suggest an inverse correlation between the fidelity and processivity properties of these enzymes. | lld:pubmed |
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| pubmed-article:9241233 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:9241233 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9241233 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9241233 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:9241233 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:9241233 | pubmed:author | pubmed-author:BackN KNK | lld:pubmed |
| pubmed-article:9241233 | pubmed:author | pubmed-author:BerkhoutBB | lld:pubmed |
| pubmed-article:9241233 | pubmed:author | pubmed-author:Oude... | lld:pubmed |
| pubmed-article:9241233 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9241233 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:9241233 | pubmed:volume | 25 | lld:pubmed |
| pubmed-article:9241233 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9241233 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9241233 | pubmed:pagination | 3212-7 | lld:pubmed |
| pubmed-article:9241233 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:9241233 | pubmed:meshHeading | pubmed-meshheading:9241233-... | lld:pubmed |
| pubmed-article:9241233 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9241233 | pubmed:articleTitle | Increased polymerase fidelity of the 3TC-resistant variants of HIV-1 reverse transcriptase. | lld:pubmed |
| pubmed-article:9241233 | pubmed:affiliation | Department of Human Retrovirology, Academic Medical Center, University of Amsterdam, PO Box 22700, 1100 DE Amsterdam, The Netherlands. | lld:pubmed |
| pubmed-article:9241233 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9241233 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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