| pubmed-article:9235968 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9235968 | lifeskim:mentions | umls-concept:C0024299 | lld:lifeskim |
| pubmed-article:9235968 | lifeskim:mentions | umls-concept:C0148873 | lld:lifeskim |
| pubmed-article:9235968 | lifeskim:mentions | umls-concept:C0205307 | lld:lifeskim |
| pubmed-article:9235968 | lifeskim:mentions | umls-concept:C0205160 | lld:lifeskim |
| pubmed-article:9235968 | lifeskim:mentions | umls-concept:C0376515 | lld:lifeskim |
| pubmed-article:9235968 | lifeskim:mentions | umls-concept:C0002085 | lld:lifeskim |
| pubmed-article:9235968 | lifeskim:mentions | umls-concept:C1704735 | lld:lifeskim |
| pubmed-article:9235968 | pubmed:issue | 31 | lld:pubmed |
| pubmed-article:9235968 | pubmed:dateCreated | 1997-8-21 | lld:pubmed |
| pubmed-article:9235968 | pubmed:abstractText | The translocated and normal bcl-2 alleles in the DHL-4 cell line with the t(14;18) translocation were separated by pulsed field electrophoresis. An in vivo footprint over a potential WT1 binding site in the bcl-2 5'-flanking sequence was identified on the normal silent allele. Electrophoretic mobility shift assays with the bcl-2 WT1 site demonstrated a single specific complex. UV cross-linking and Western analysis revealed that this gel shift complex contained WT1 protein. Deletion or mutation of the WT1 site resulted in an increase in activity of the bcl-2 promoter in DHL-4 cells. Cotransfection with a 3:1 ratio of a WT1 expression vector to the bcl-2 promoter construct led to a 3.0-fold repression of the bcl-2 promoter. Cotransfection with a WT1 expression vector and the bcl-2 promoter with the mutated WT1 site resulted in only 1.2-fold repression. We conclude that the WT1 site functions as a negative regulatory site for the normal silent bcl-2 allele in t(14;18) lymphomas. The WT1 site is not occupied on the translocated bcl-2 allele. | lld:pubmed |
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| pubmed-article:9235968 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9235968 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9235968 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9235968 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9235968 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9235968 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:9235968 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:9235968 | pubmed:author | pubmed-author:HeckmanCC | lld:pubmed |
| pubmed-article:9235968 | pubmed:author | pubmed-author:BoxerL MLM | lld:pubmed |
| pubmed-article:9235968 | pubmed:author | pubmed-author:ArcinasMM | lld:pubmed |
| pubmed-article:9235968 | pubmed:author | pubmed-author:MochonEE | lld:pubmed |
| pubmed-article:9235968 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9235968 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:9235968 | pubmed:volume | 272 | lld:pubmed |
| pubmed-article:9235968 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9235968 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9235968 | pubmed:pagination | 19609-14 | lld:pubmed |
| pubmed-article:9235968 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:9235968 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9235968 | pubmed:articleTitle | The WT1 protein is a negative regulator of the normal bcl-2 allele in t(14;18) lymphomas. | lld:pubmed |
| pubmed-article:9235968 | pubmed:affiliation | Center for Molecular Biology in Medicine, Palo Alto Veterans Administration Medical Center, Stanford, California 94305, USA. | lld:pubmed |
| pubmed-article:9235968 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9235968 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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