| pubmed-article:9234892 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9234892 | lifeskim:mentions | umls-concept:C0034307 | lld:lifeskim |
| pubmed-article:9234892 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:9234892 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:9234892 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:9234892 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:9234892 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:9234892 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:9234892 | pubmed:dateCreated | 1997-9-4 | lld:pubmed |
| pubmed-article:9234892 | pubmed:abstractText | Two fluorimetric assays for the determination of pyroglutamyl aminopeptidase type-II activity have been developed. The assays are based on hydrolysis of the quenched-fluorimetric substrate <Glu-His-Pro-7-amino-4-methylcoumarin. Following the removal of the N-terminal <Glu by pyroglutamyl aminopeptidase type-II, liberation of 7-amino-4-methylcoumarin from the metabolite His-Pro-7-amino-4-methylcoumarin is catalyzed by one of two methods: (i) the addition of partially purified bovine serum dipeptidyl aminopeptidase type-IV or (ii) by incubating the reaction mixture for up to 2 h at 80 degrees C, thus promoting the nonenzymatic cyclization of His-Pro-7-amino-4-methylcoumarin to cyclo His-Pro and free 7-amino-4-methylcoumarin. Pyroglutamyl aminopeptidase type-II from bovine brain is used to establish appropriate assay conditions. These fluorimetric assays offer expeditious alternatives to the existing radiolabeled thyrotropin-releasing hormone assays for the determination of PAPII activity. | lld:pubmed |
| pubmed-article:9234892 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9234892 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9234892 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9234892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9234892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9234892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9234892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9234892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9234892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9234892 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9234892 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9234892 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:9234892 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:9234892 | pubmed:author | pubmed-author:O'ConnorBB | lld:pubmed |
| pubmed-article:9234892 | pubmed:author | pubmed-author:O'LearyR MRM | lld:pubmed |
| pubmed-article:9234892 | pubmed:author | pubmed-author:GallagherS... | lld:pubmed |
| pubmed-article:9234892 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9234892 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:9234892 | pubmed:volume | 250 | lld:pubmed |
| pubmed-article:9234892 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9234892 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9234892 | pubmed:pagination | 1-9 | lld:pubmed |
| pubmed-article:9234892 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:9234892 | pubmed:meshHeading | pubmed-meshheading:9234892-... | lld:pubmed |
| pubmed-article:9234892 | pubmed:meshHeading | pubmed-meshheading:9234892-... | lld:pubmed |
| pubmed-article:9234892 | pubmed:meshHeading | pubmed-meshheading:9234892-... | lld:pubmed |
| pubmed-article:9234892 | pubmed:meshHeading | pubmed-meshheading:9234892-... | lld:pubmed |
| pubmed-article:9234892 | pubmed:meshHeading | pubmed-meshheading:9234892-... | lld:pubmed |
| pubmed-article:9234892 | pubmed:meshHeading | pubmed-meshheading:9234892-... | lld:pubmed |
| pubmed-article:9234892 | pubmed:meshHeading | pubmed-meshheading:9234892-... | lld:pubmed |
| pubmed-article:9234892 | pubmed:meshHeading | pubmed-meshheading:9234892-... | lld:pubmed |
| pubmed-article:9234892 | pubmed:meshHeading | pubmed-meshheading:9234892-... | lld:pubmed |
| pubmed-article:9234892 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9234892 | pubmed:articleTitle | The development of two fluorimetric assays for the determination of pyroglutamyl aminopeptidase type-II activity. | lld:pubmed |
| pubmed-article:9234892 | pubmed:affiliation | School of Biological Sciences, Dublin City University, Glasnevin, Ireland. | lld:pubmed |
| pubmed-article:9234892 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9234892 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
