| pubmed-article:9211424 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9211424 | lifeskim:mentions | umls-concept:C0013935 | lld:lifeskim |
| pubmed-article:9211424 | lifeskim:mentions | umls-concept:C0043457 | lld:lifeskim |
| pubmed-article:9211424 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:9211424 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:9211424 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:9211424 | lifeskim:mentions | umls-concept:C0086222 | lld:lifeskim |
| pubmed-article:9211424 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:9211424 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:9211424 | lifeskim:mentions | umls-concept:C1705733 | lld:lifeskim |
| pubmed-article:9211424 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:9211424 | pubmed:dateCreated | 1997-8-18 | lld:pubmed |
| pubmed-article:9211424 | pubmed:abstractText | The transient expression of reporter gene constructs in embryos provides a powerful tool to characterise cis-acting transcriptional elements of the genes involved in development. In the present study, we have analysed the expression pattern of several muscle-specific and ubiquitous regulatory sequences in microinjected zebrafish embryos. By using a fast and reproducible coinjection strategy, the mosaic expression of lacZ reporter gene was monitored in wholemount embryos injected with sequences containing putative enhancer elements and a carp myosin heavy chain promoter/lacZ reporter construct. We have found that a 0.9-kb myosin heavy chain (MyHC) proximal promoter containing several putative myogenic regulatory factors (MRF) binding sites is sufficient to restrict lacZ expression to the skeletal muscle fibres of prim-6 stage zebrafish embryos. Expression of a rat-derived foetal myosin light chain enhancer (MyLC) and different fragments of a carp beta-actin regulatory region together with the MyHC promoter were compared by accumulating the type, number and spatial distribution of beta-galactosidase-expressing cells on an expression map. beta-galactosidase activity increased similarly whether the MyLC enhancer was ligated to the promoter/ reporter construct directly or when coinjected as a separate fragment whilst skeletal muscle specificity was retained. The coinjection of two different forms of the beta-actin regulatory elements also showed a marked effect on the MyHC promoter activity. The coinjection of putative enhancers with minimal promoter constructs and subsequent analysis of the transient expression pattern in the developing embryos provides a rapid and simple technique to identify cis acting activator elements of genes expressed in the vertebrate embryo. | lld:pubmed |
| pubmed-article:9211424 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9211424 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9211424 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9211424 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9211424 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9211424 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9211424 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9211424 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9211424 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:9211424 | pubmed:issn | 1040-452X | lld:pubmed |
| pubmed-article:9211424 | pubmed:author | pubmed-author:OrbánLL | lld:pubmed |
| pubmed-article:9211424 | pubmed:author | pubmed-author:MüllerFF | lld:pubmed |
| pubmed-article:9211424 | pubmed:author | pubmed-author:GoldspinkGG | lld:pubmed |
| pubmed-article:9211424 | pubmed:author | pubmed-author:MacleanNN | lld:pubmed |
| pubmed-article:9211424 | pubmed:author | pubmed-author:WilliamsD WDW | lld:pubmed |
| pubmed-article:9211424 | pubmed:author | pubmed-author:GauvryLL | lld:pubmed |
| pubmed-article:9211424 | pubmed:author | pubmed-author:KobolákJJ | lld:pubmed |
| pubmed-article:9211424 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9211424 | pubmed:volume | 47 | lld:pubmed |
| pubmed-article:9211424 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9211424 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9211424 | pubmed:pagination | 404-12 | lld:pubmed |
| pubmed-article:9211424 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:9211424 | pubmed:meshHeading | pubmed-meshheading:9211424-... | lld:pubmed |
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| pubmed-article:9211424 | pubmed:meshHeading | pubmed-meshheading:9211424-... | lld:pubmed |
| pubmed-article:9211424 | pubmed:meshHeading | pubmed-meshheading:9211424-... | lld:pubmed |
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| pubmed-article:9211424 | pubmed:meshHeading | pubmed-meshheading:9211424-... | lld:pubmed |
| pubmed-article:9211424 | pubmed:meshHeading | pubmed-meshheading:9211424-... | lld:pubmed |
| pubmed-article:9211424 | pubmed:meshHeading | pubmed-meshheading:9211424-... | lld:pubmed |
| pubmed-article:9211424 | pubmed:meshHeading | pubmed-meshheading:9211424-... | lld:pubmed |
| pubmed-article:9211424 | pubmed:meshHeading | pubmed-meshheading:9211424-... | lld:pubmed |
| pubmed-article:9211424 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9211424 | pubmed:articleTitle | Activator effect of coinjected enhancers on the muscle-specific expression of promoters in zebrafish embryos. | lld:pubmed |
| pubmed-article:9211424 | pubmed:affiliation | Institute for Molecular Genetics, Agricultural Biotechnology Center, Gödöllö, Hungary. | lld:pubmed |
| pubmed-article:9211424 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9211424 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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