| pubmed-article:9207439 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9207439 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
| pubmed-article:9207439 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:9207439 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:9207439 | lifeskim:mentions | umls-concept:C0023473 | lld:lifeskim |
| pubmed-article:9207439 | lifeskim:mentions | umls-concept:C2323499 | lld:lifeskim |
| pubmed-article:9207439 | lifeskim:mentions | umls-concept:C0205307 | lld:lifeskim |
| pubmed-article:9207439 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:9207439 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:9207439 | pubmed:dateCreated | 1997-7-22 | lld:pubmed |
| pubmed-article:9207439 | pubmed:abstractText | We have previously reported that primitive normal hematopoietic cells detectable as long-term culture-initiating cells (Ph-LTC-IC) are present at high levels in the blood of some patients with chronic myeloid leukemia (CML). We now show that this population can be expanded several-fold when highly purified CD34+CD38- cells isolated from the blood of such patients are cultured for 10 days in a serum-free medium containing 100 ng/mL of Flt3-ligand and Steel factor and 20 ng/mL of interleukin-3 (IL-3) and IL-6, and granulocyte colony-stimulating factor. In similar cultures initiated with CD34+CD38- cells from CML blood samples in which all of the LTC-IC were leukemic (Ph+), Ph+ LTC-IC activity was rapidly lost both in the presence and absence of admixed CD34+CD38- cells isolated from normal marrow. Conversely, the ability of normal LTC-IC to expand their numbers was shown to be independent of the presence of Ph+LTC-IC and later types of Ph+colony-forming cell (CFC) progenitors. In contrast to the LTC-IC, CFC were consistently amplified in cultures initiated with CML-derived CD34+CD38- cells and the additional CFC present after 10 days were, like the starting population of CFC, almost exclusively Ph+ regardless of the genotype(s) of the LTC-IC in the original CML samples. Amplification of the Ph+CFC population in these cultures showed the same factor dependence as previously demonstrated for the in vitro expansion of CFC from normal marrow CD34+CD38- cells. Ph+LTC-IC disappeared regardless of the cytokines present. Taken together these findings support a model of CML in which the leukemic stem cells are characterized by a decreased probability of self-renewal and an increased probability of differentiation. In addition, they suggest new opportunities for improving the treatment of CML using strategies that require autologous stem cell rescue. | lld:pubmed |
| pubmed-article:9207439 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9207439 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9207439 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:9207439 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9207439 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9207439 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9207439 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9207439 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9207439 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9207439 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:9207439 | pubmed:issn | 0006-4971 | lld:pubmed |
| pubmed-article:9207439 | pubmed:author | pubmed-author:EavesA CAC | lld:pubmed |
| pubmed-article:9207439 | pubmed:author | pubmed-author:EavesC JCJ | lld:pubmed |
| pubmed-article:9207439 | pubmed:author | pubmed-author:BarnettM JMJ | lld:pubmed |
| pubmed-article:9207439 | pubmed:author | pubmed-author:PetzerA LAL | lld:pubmed |
| pubmed-article:9207439 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9207439 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:9207439 | pubmed:volume | 90 | lld:pubmed |
| pubmed-article:9207439 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9207439 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9207439 | pubmed:pagination | 64-9 | lld:pubmed |
| pubmed-article:9207439 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:9207439 | pubmed:meshHeading | pubmed-meshheading:9207439-... | lld:pubmed |
| pubmed-article:9207439 | pubmed:meshHeading | pubmed-meshheading:9207439-... | lld:pubmed |
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| pubmed-article:9207439 | pubmed:meshHeading | pubmed-meshheading:9207439-... | lld:pubmed |
| pubmed-article:9207439 | pubmed:meshHeading | pubmed-meshheading:9207439-... | lld:pubmed |
| pubmed-article:9207439 | pubmed:meshHeading | pubmed-meshheading:9207439-... | lld:pubmed |
| pubmed-article:9207439 | pubmed:meshHeading | pubmed-meshheading:9207439-... | lld:pubmed |
| pubmed-article:9207439 | pubmed:meshHeading | pubmed-meshheading:9207439-... | lld:pubmed |
| pubmed-article:9207439 | pubmed:meshHeading | pubmed-meshheading:9207439-... | lld:pubmed |
| pubmed-article:9207439 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9207439 | pubmed:articleTitle | Selective expansion of primitive normal hematopoietic cells in cytokine-supplemented cultures of purified cells from patients with chronic myeloid leukemia. | lld:pubmed |
| pubmed-article:9207439 | pubmed:affiliation | British Columbia Cancer Agency, Department of Medical Genetics, University of British Columbia, Vancouver, Canada. | lld:pubmed |
| pubmed-article:9207439 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9207439 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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