| pubmed-article:9200466 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9200466 | lifeskim:mentions | umls-concept:C0022567 | lld:lifeskim |
| pubmed-article:9200466 | lifeskim:mentions | umls-concept:C0383327 | lld:lifeskim |
| pubmed-article:9200466 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:9200466 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:9200466 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:9200466 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:9200466 | lifeskim:mentions | umls-concept:C0591833 | lld:lifeskim |
| pubmed-article:9200466 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:9200466 | pubmed:dateCreated | 1997-7-14 | lld:pubmed |
| pubmed-article:9200466 | pubmed:abstractText | Recently, the novel cytokine IL-18 (IFN-gamma-inducing factor) has been described as a growth and differentiation factor for Th1 cells. Epidermal keratinocytes (KC) are known to direct T cell education by production of cytokines. Therefore, expression of IL-18 was sought in KC. Epidermal RNA was analyzed following stimulation with contact sensitizers or controls for IL-18 mRNA expression by semiquantitative reverse transcription-PCR. Constitutive expression of IL-18 mRNA was low in untreated epidermal cells (EC), but early up-regulation of IL-18 mRNA signals was detected following application of a contact allergen in vivo. The peak strength of IL-18 signals was observed within 4 to 6 h following stimulation with an allergen. Application of an irritant (benzalconiumchloride) or solvents did not result in increased signal strength. To determine the cellular origin of IL-18 mRNA in EC, depletion experiments were performed. IL-18 signals were not affected by depletion with anti-CD3 (T cells) or anti-MHC class II mAb-coupled beads identifying KC as a major source of IL-18. These results were confirmed by analysis of mRNA derived from the KC cell line PAM 212. Strong IL-18 signals could be detected by reverse transcription-PCR. To delineate whether IL-18 protein was produced by EC/KC, a sandwich ELISA was used to assay for IL-18 production. Supernatants from allergen-stimulated EC and KC showed production of IL-18 protein. To confirm that IL-18 protein was functional, EC or KC supernatants were tested for their ability to induce IFN-gamma production. Significant amounts of IFN-gamma were detected in supernatants of allergen-treated cells. In aggregate, our data indicate that murine KC are a source of both IL-18 mRNA and functional protein. | lld:pubmed |
| pubmed-article:9200466 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9200466 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9200466 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:9200466 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9200466 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9200466 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9200466 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9200466 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9200466 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:9200466 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:9200466 | pubmed:author | pubmed-author:OkamuraHH | lld:pubmed |
| pubmed-article:9200466 | pubmed:author | pubmed-author:TanimotoTT | lld:pubmed |
| pubmed-article:9200466 | pubmed:author | pubmed-author:MüllerGG | lld:pubmed |
| pubmed-article:9200466 | pubmed:author | pubmed-author:KnoxBB | lld:pubmed |
| pubmed-article:9200466 | pubmed:author | pubmed-author:YamauchiHH | lld:pubmed |
| pubmed-article:9200466 | pubmed:author | pubmed-author:StollSS | lld:pubmed |
| pubmed-article:9200466 | pubmed:author | pubmed-author:KurimotoMM | lld:pubmed |
| pubmed-article:9200466 | pubmed:author | pubmed-author:EnkA HAH | lld:pubmed |
| pubmed-article:9200466 | pubmed:author | pubmed-author:SalogaJJ | lld:pubmed |
| pubmed-article:9200466 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9200466 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:9200466 | pubmed:volume | 159 | lld:pubmed |
| pubmed-article:9200466 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9200466 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9200466 | pubmed:pagination | 298-302 | lld:pubmed |
| pubmed-article:9200466 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:9200466 | pubmed:meshHeading | pubmed-meshheading:9200466-... | lld:pubmed |
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| pubmed-article:9200466 | pubmed:meshHeading | pubmed-meshheading:9200466-... | lld:pubmed |
| pubmed-article:9200466 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9200466 | pubmed:articleTitle | Production of IL-18 (IFN-gamma-inducing factor) messenger RNA and functional protein by murine keratinocytes. | lld:pubmed |
| pubmed-article:9200466 | pubmed:affiliation | Department of Dermatology, University of Mainz, Germany. | lld:pubmed |
| pubmed-article:9200466 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9200466 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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