| pubmed-article:9179290 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9179290 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:9179290 | lifeskim:mentions | umls-concept:C0162605 | lld:lifeskim |
| pubmed-article:9179290 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:9179290 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:9179290 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:9179290 | lifeskim:mentions | umls-concept:C0065561 | lld:lifeskim |
| pubmed-article:9179290 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:9179290 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:9179290 | pubmed:dateCreated | 1997-7-14 | lld:pubmed |
| pubmed-article:9179290 | pubmed:abstractText | The cDNA encoding the 65-kDa subunit of malic enzyme from Ascaris suum was cloned into the bacterial expression vector pKK223-3 and overproduced in Escherichia coli. A protein with a subunit molecular mass of 65,000 was expressed at a level of up to 3% of the total soluble protein in JM109, as judged by SDS-PAGE. The enzyme was purified using column chromatography on phenyl-Sepharose followed by orange-A agarose. The purification procedure resulted in a 32-fold purification with an overall yield of 51%. The bacterially expressed enzyme exhibits kinetic constants identical to those measured for native A. suum NAD-malic enzyme. | lld:pubmed |
| pubmed-article:9179290 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9179290 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9179290 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9179290 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9179290 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9179290 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9179290 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9179290 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9179290 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9179290 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9179290 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:9179290 | pubmed:issn | 1046-5928 | lld:pubmed |
| pubmed-article:9179290 | pubmed:author | pubmed-author:HarrisB GBG | lld:pubmed |
| pubmed-article:9179290 | pubmed:author | pubmed-author:CookP FPF | lld:pubmed |
| pubmed-article:9179290 | pubmed:author | pubmed-author:KarstenW EWE | lld:pubmed |
| pubmed-article:9179290 | pubmed:author | pubmed-author:KulkarniGG | lld:pubmed |
| pubmed-article:9179290 | pubmed:author | pubmed-author:NalaboluS RSR | lld:pubmed |
| pubmed-article:9179290 | pubmed:author | pubmed-author:ChoobackLL | lld:pubmed |
| pubmed-article:9179290 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9179290 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:9179290 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9179290 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9179290 | pubmed:pagination | 51-4 | lld:pubmed |
| pubmed-article:9179290 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:9179290 | pubmed:meshHeading | pubmed-meshheading:9179290-... | lld:pubmed |
| pubmed-article:9179290 | pubmed:meshHeading | pubmed-meshheading:9179290-... | lld:pubmed |
| pubmed-article:9179290 | pubmed:meshHeading | pubmed-meshheading:9179290-... | lld:pubmed |
| pubmed-article:9179290 | pubmed:meshHeading | pubmed-meshheading:9179290-... | lld:pubmed |
| pubmed-article:9179290 | pubmed:meshHeading | pubmed-meshheading:9179290-... | lld:pubmed |
| pubmed-article:9179290 | pubmed:meshHeading | pubmed-meshheading:9179290-... | lld:pubmed |
| pubmed-article:9179290 | pubmed:meshHeading | pubmed-meshheading:9179290-... | lld:pubmed |
| pubmed-article:9179290 | pubmed:meshHeading | pubmed-meshheading:9179290-... | lld:pubmed |
| pubmed-article:9179290 | pubmed:meshHeading | pubmed-meshheading:9179290-... | lld:pubmed |
| pubmed-article:9179290 | pubmed:meshHeading | pubmed-meshheading:9179290-... | lld:pubmed |
| pubmed-article:9179290 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9179290 | pubmed:articleTitle | Expression, purification, and characterization of the recombinant NAD-malic enzyme from Ascaris suum. | lld:pubmed |
| pubmed-article:9179290 | pubmed:affiliation | Department of Biochemistry and Molecular Biology, University of North Texas Health Science Center at Fort Worth 76107-2699, USA. | lld:pubmed |
| pubmed-article:9179290 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9179290 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
