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pubmed-article:9177306pubmed:abstractTextExpression of endothelin (ET) receptor subtypes was examined in an experimental model of A375 human melanoma cell differentiation using the pyrimidine analog bromodeoxyuridine (BUdR). BUdR (10 microM)-treated cells had an increased surface area and an increased dendricity, were contact-inhibited and lacked tumorigenecity in athymic nude mice. The untreated A375 cells exclusively expressed ETB and BUdR-induced phenotypical changes were accompanied by induction of ETA expression as evidenced by northern blotting, [125I]ET-1 binding assay and [Ca2+]i measurement. Thus, BUdR-induced differentiation of A375 melanoma cells may provide a model system to study the receptor subtype switch in melanocyte development.lld:pubmed
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pubmed-article:9177306pubmed:articleTitleBromodeoxyuridine-induced expression of endothelin A in A375 human melanoma cells.lld:pubmed
pubmed-article:9177306pubmed:affiliationDepartment of Pharmacology, Faculty of Medicine, Kyoto University, Japan.lld:pubmed
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