| pubmed-article:9172423 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9172423 | lifeskim:mentions | umls-concept:C0008633 | lld:lifeskim |
| pubmed-article:9172423 | lifeskim:mentions | umls-concept:C0058075 | lld:lifeskim |
| pubmed-article:9172423 | lifeskim:mentions | umls-concept:C0023276 | lld:lifeskim |
| pubmed-article:9172423 | lifeskim:mentions | umls-concept:C0599777 | lld:lifeskim |
| pubmed-article:9172423 | pubmed:dateCreated | 1997-7-21 | lld:pubmed |
| pubmed-article:9172423 | pubmed:abstractText | We have used a chromosome-specific approach to generate a 300 kb long 'contig' across Leishmania major 500 kb chromosome. Clones from a 13-hit genomic library served as templates to generate end-specific probes that were used in hybridization to a high density array of the library. The 'contig' generated contained 12 markers uniformly spaced. Three restriction endonucleases were mapped within the map extending its resolution. Map extension indicated a peculiar feature of sequence organization in subtelomeric regions where chromosome-specificity of mapping is lost. End-probes generated from clones mapping to the extremes of a 300 kb 'contig' rescued a high percentage of 2 types of clones from the genomic library, 1 of which showed positive hybridization to the hexameric telomere repeat. Fine mapping at these regions revealed that these 2 clones contained elements common to all chromosomes of the parasite. The physical map generated constitutes ready-to-use data for the study of many aspects of genome organization. Being cloned in a shuttle vector, the genomic sequences reordered in the map can be used to generate genetic information by transfection into the parasite. | lld:pubmed |
| pubmed-article:9172423 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9172423 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9172423 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9172423 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9172423 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9172423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9172423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9172423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9172423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9172423 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9172423 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9172423 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:9172423 | pubmed:issn | 0031-1820 | lld:pubmed |
| pubmed-article:9172423 | pubmed:author | pubmed-author:BeverleyS MSM | lld:pubmed |
| pubmed-article:9172423 | pubmed:author | pubmed-author:CruzA KAK | lld:pubmed |
| pubmed-article:9172423 | pubmed:author | pubmed-author:CasagrandeLL | lld:pubmed |
| pubmed-article:9172423 | pubmed:author | pubmed-author:TosiL RLR | lld:pubmed |
| pubmed-article:9172423 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9172423 | pubmed:volume | 114 ( Pt 6) | lld:pubmed |
| pubmed-article:9172423 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9172423 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9172423 | pubmed:pagination | 521-9 | lld:pubmed |
| pubmed-article:9172423 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:9172423 | pubmed:meshHeading | pubmed-meshheading:9172423-... | lld:pubmed |
| pubmed-article:9172423 | pubmed:meshHeading | pubmed-meshheading:9172423-... | lld:pubmed |
| pubmed-article:9172423 | pubmed:meshHeading | pubmed-meshheading:9172423-... | lld:pubmed |
| pubmed-article:9172423 | pubmed:meshHeading | pubmed-meshheading:9172423-... | lld:pubmed |
| pubmed-article:9172423 | pubmed:meshHeading | pubmed-meshheading:9172423-... | lld:pubmed |
| pubmed-article:9172423 | pubmed:meshHeading | pubmed-meshheading:9172423-... | lld:pubmed |
| pubmed-article:9172423 | pubmed:meshHeading | pubmed-meshheading:9172423-... | lld:pubmed |
| pubmed-article:9172423 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9172423 | pubmed:articleTitle | Physical mapping across the dihydrofolate reductase-thymidylate synthase chromosome of Leishmania major. | lld:pubmed |
| pubmed-article:9172423 | pubmed:affiliation | Departamento de Bioquimica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brasil. | lld:pubmed |
| pubmed-article:9172423 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9172423 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9172423 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
