| pubmed-article:9160667 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9160667 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:9160667 | lifeskim:mentions | umls-concept:C0038250 | lld:lifeskim |
| pubmed-article:9160667 | lifeskim:mentions | umls-concept:C0018951 | lld:lifeskim |
| pubmed-article:9160667 | lifeskim:mentions | umls-concept:C1517488 | lld:lifeskim |
| pubmed-article:9160667 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:9160667 | pubmed:dateCreated | 1997-6-19 | lld:pubmed |
| pubmed-article:9160667 | pubmed:abstractText | The microenvironment is a key regulator of hematopoietic stem cells (HSCs) and is a likely source of extracellular factors that control stem cell fate. A better understanding of these microenvironmental factors may come from investigations of developmental cell fate determination in which the critical roles of cell-cell interactions of multipotential cells have been shown. The Wnt gene family is known to regulate the cell fate and cell-cell interactions of multipotential cells in a variety of tissues. Expression of Wnts and of their putative receptors encoded by murine homologs of the Drosophila frizzled gene in hematopoietic tissues was examined by reverse transcriptase-polymerase chain reaction. Wnt-5a and Wnt-10b were expressed in day-11 murine yolk sac, day-14 fetal liver, and fetal liver AA4+ cells. The expression profiles of four murine frizzled homologs, Mfz3-7, were nearly identical to that of Wnt-5a and Wnt-10b. Notably, Wnt-10b was expressed in the fetal liver AA4+ Sca+ c-kit+ flASK) HSC population. A role for Wnts in HSC fate determination was studied by treatment of HSC populations in culture with soluble WNT proteins. The addition of conditioned media from cells transfected with Wnt-1, Wnt-5a, or Wnt-10b cDNAs to cultures of flASK cells stimulated a sevenfold, eightfold, and 11-fold expansion in cell number, respectively, relative to control media. Removal of WNT-5a from this media by immunodepletion depleted the stimulatory activity from the media, whereas addition of a partially purified WNT-5a stimulated a fivefold expansion relative to control cells. Transduction of flASK cells with a retrovirus bearing a Wnt-5a cDNA enhanced proliferation. We conclude that WNTs stimulate the survival/proliferation of hematopoietic progenitors, demonstrating that WNTs comprise a novel class of hematopoietic cell regulators. | lld:pubmed |
| pubmed-article:9160667 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9160667 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:9160667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9160667 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9160667 | pubmed:month | May | lld:pubmed |
| pubmed-article:9160667 | pubmed:issn | 0006-4971 | lld:pubmed |
| pubmed-article:9160667 | pubmed:author | pubmed-author:AustinT WTW | lld:pubmed |
| pubmed-article:9160667 | pubmed:author | pubmed-author:MatthewsWW | lld:pubmed |
| pubmed-article:9160667 | pubmed:author | pubmed-author:NuttJ DJD | lld:pubmed |
| pubmed-article:9160667 | pubmed:author | pubmed-author:SolarG PGP | lld:pubmed |
| pubmed-article:9160667 | pubmed:author | pubmed-author:ZieglerF CFC | lld:pubmed |
| pubmed-article:9160667 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9160667 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:9160667 | pubmed:volume | 89 | lld:pubmed |
| pubmed-article:9160667 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9160667 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9160667 | pubmed:pagination | 3624-35 | lld:pubmed |
| pubmed-article:9160667 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:meshHeading | pubmed-meshheading:9160667-... | lld:pubmed |
| pubmed-article:9160667 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9160667 | pubmed:articleTitle | A role for the Wnt gene family in hematopoiesis: expansion of multilineage progenitor cells. | lld:pubmed |
| pubmed-article:9160667 | pubmed:affiliation | Department of Molecular Oncology, Genentech, Inc, South San Francisco, CA 94080, USA. | lld:pubmed |
| pubmed-article:9160667 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:14365 | entrezgene:pubmed | pubmed-article:9160667 | lld:entrezgene |
| entrez-gene:14366 | entrezgene:pubmed | pubmed-article:9160667 | lld:entrezgene |
| entrez-gene:14367 | entrezgene:pubmed | pubmed-article:9160667 | lld:entrezgene |
| entrez-gene:14368 | entrezgene:pubmed | pubmed-article:9160667 | lld:entrezgene |
| entrez-gene:14369 | entrezgene:pubmed | pubmed-article:9160667 | lld:entrezgene |
| entrez-gene:22408 | entrezgene:pubmed | pubmed-article:9160667 | lld:entrezgene |
| entrez-gene:22410 | entrezgene:pubmed | pubmed-article:9160667 | lld:entrezgene |
| entrez-gene:22416 | entrezgene:pubmed | pubmed-article:9160667 | lld:entrezgene |
| entrez-gene:22418 | entrezgene:pubmed | pubmed-article:9160667 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9160667 | lld:pubmed |
