| pubmed-article:9143286 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9143286 | lifeskim:mentions | umls-concept:C0042776 | lld:lifeskim |
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| pubmed-article:9143286 | lifeskim:mentions | umls-concept:C0009450 | lld:lifeskim |
| pubmed-article:9143286 | lifeskim:mentions | umls-concept:C0009013 | lld:lifeskim |
| pubmed-article:9143286 | lifeskim:mentions | umls-concept:C0080194 | lld:lifeskim |
| pubmed-article:9143286 | lifeskim:mentions | umls-concept:C0006556 | lld:lifeskim |
| pubmed-article:9143286 | lifeskim:mentions | umls-concept:C0042210 | lld:lifeskim |
| pubmed-article:9143286 | lifeskim:mentions | umls-concept:C0332161 | lld:lifeskim |
| pubmed-article:9143286 | lifeskim:mentions | umls-concept:C1550587 | lld:lifeskim |
| pubmed-article:9143286 | lifeskim:mentions | umls-concept:C1527177 | lld:lifeskim |
| pubmed-article:9143286 | lifeskim:mentions | umls-concept:C0441513 | lld:lifeskim |
| pubmed-article:9143286 | lifeskim:mentions | umls-concept:C1527240 | lld:lifeskim |
| pubmed-article:9143286 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:9143286 | pubmed:dateCreated | 1997-6-5 | lld:pubmed |
| pubmed-article:9143286 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9143286 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9143286 | pubmed:abstractText | We identified nine nucleotide differences between the genomes of dengue-2 (DEN-2) 16681 virus and its vaccine derivative, strain PDK-53. These included a C-to-T (16681-to-PDK-53) mutation at nucleotide position 57 of the 5'-untranslated region, three silent mutations, and substitutions prM-29 Asp to Val, NS1-53 Gly to Asp, NS2A-181 Leu to Phe, NS3-250 Glu to Val, and NS4A-75 Gly to Ala. Unpassaged PDK-53 vaccine contained two genetic variants as a result of partial mutation at NS3-250. We constructed infectious cDNA clones for 16681 virus and each of the two PDK-53 variants. DEN-2 16681 clone-derived viruses were identical to the 16681 virus in plaque size and replication in LLC-MK2 cells, replication in C6/36 cells, E and prM epitopes, and neurovirulence for suckling mice. PDK-53 virus and both clone-derived PDK-53 variants were attenuated in mice. However, the variant containing NS3-250-Glu was less temperature sensitive and replicated better in C6/36 cells than did PDK-53 virus. The variant containing NS3-250-Val had smaller, more diffuse plaques, decreased replication, and increased temperature sensitivity in LLC-MK2 cells relative to PDK-53 virus. Both PDK-53 virus and the NS3-250-Val variant replicated poorly in C6/36 cells relative to 16681 virus. Unpassaged PDK-53 vaccine virus and the virus passaged once in LLC-MK2 cells had genomes of identical sequence, including the mixed NS3-250-Glu/Val locus. Although the NS3-250-Val mutation clearly affected virus replication in vitro, it was not a major determinant of attenuation for PDK-53 virus in suckling mice. | lld:pubmed |
| pubmed-article:9143286 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9143286 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9143286 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9143286 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9143286 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:9143286 | pubmed:issn | 0042-6822 | lld:pubmed |
| pubmed-article:9143286 | pubmed:author | pubmed-author:GublerD JDJ | lld:pubmed |
| pubmed-article:9143286 | pubmed:author | pubmed-author:Bhamarapravat... | lld:pubmed |
| pubmed-article:9143286 | pubmed:author | pubmed-author:TsuchiyaK RKR | lld:pubmed |
| pubmed-article:9143286 | pubmed:author | pubmed-author:RoehrigJ TJT | lld:pubmed |
| pubmed-article:9143286 | pubmed:author | pubmed-author:ChangG JGJ | lld:pubmed |
| pubmed-article:9143286 | pubmed:author | pubmed-author:KinneyR MRM | lld:pubmed |
| pubmed-article:9143286 | pubmed:author | pubmed-author:ButrapetSS | lld:pubmed |
| pubmed-article:9143286 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9143286 | pubmed:day | 14 | lld:pubmed |
| pubmed-article:9143286 | pubmed:volume | 230 | lld:pubmed |
| pubmed-article:9143286 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9143286 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9143286 | pubmed:pagination | 300-8 | lld:pubmed |
| pubmed-article:9143286 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:9143286 | pubmed:meshHeading | pubmed-meshheading:9143286-... | lld:pubmed |
| pubmed-article:9143286 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9143286 | pubmed:articleTitle | Construction of infectious cDNA clones for dengue 2 virus: strain 16681 and its attenuated vaccine derivative, strain PDK-53. | lld:pubmed |
| pubmed-article:9143286 | pubmed:affiliation | Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA. rmk1@cdc.gov | lld:pubmed |
| pubmed-article:9143286 | pubmed:publicationType | Journal Article | lld:pubmed |
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