| pubmed-article:9096351 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9096351 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:9096351 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:9096351 | lifeskim:mentions | umls-concept:C0013935 | lld:lifeskim |
| pubmed-article:9096351 | lifeskim:mentions | umls-concept:C0221908 | lld:lifeskim |
| pubmed-article:9096351 | lifeskim:mentions | umls-concept:C0008524 | lld:lifeskim |
| pubmed-article:9096351 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:9096351 | lifeskim:mentions | umls-concept:C1565824 | lld:lifeskim |
| pubmed-article:9096351 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:9096351 | lifeskim:mentions | umls-concept:C1414683 | lld:lifeskim |
| pubmed-article:9096351 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:9096351 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:9096351 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:9096351 | pubmed:dateCreated | 1997-5-8 | lld:pubmed |
| pubmed-article:9096351 | pubmed:abstractText | Mammalian hepatocyte nuclear factor-3 (HNF-3) and the Drosophila homeotic gene fork head proteins are prototypes of an extensive family of cell-specific transcription factors that share homology in the winged helix DNA-binding domain. One of these mammalian family members, HNF-3/fork head homolog-4 (HFH-4), was isolated by PCR amplification of rodent brain cDNA and exhibits abundant expression in the adult bronchiolar epithelium. In this study, we performed in situ hybridization of stage-specific mouse embryos and report on a novel expression pattern of the HFH-4 gene in both the presumptive and differentiated choroid plexus epithelium, which is responsible for the synthesis and secretion of cerebrospinal fluid (CSF) proteins. We also showed that HFH-4 is a potent transcriptional activator in cotransfection assays and defined several protein sequences important for HFH-4 transcriptional activity. We used in vitro DNA-binding site selection with recombinant HFH-4 protein and determined that the HFH-4 protein recognizes the DNA consensus sequences HWDTGTTTGTTTA or KTTTGTTGTTKTW (where H is not G, W is A or T, D is not C, and K is G or T). We used this HFH-4 consensus to identify potential HFH-4 target genes in the choroid plexus epithelium and demonstrated that these promoter sequences bind to recombinant HFH-4 protein in electrophoretic mobility shift assays. Recombinant HFH-4 formed specific protein-DNA complexes with the promoter regions of the human prothrombin, beta amyloid precursor protein, alpha1-antichymotrypsin, cystic fibrosis transmembrane conductance regulator and rodent alpha2-macroglobulin, growth hormone receptors, and insulin-like growth factor II genes. Furthermore, we identified putative HFH-4 target genes in the bronchiolar epithelium including the clara cell secretory protein gene and the HNF-3alpha gene, a winged helix family member involved in the transcriptional regulation of genes in the bronchiolar epithelium. In support of these binding studies, cotransfection assays show that HFH-4 potentiates expression of the HNF-3alpha and clara cell secretory protein promoter regions. | lld:pubmed |
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| pubmed-article:9096351 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9096351 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9096351 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9096351 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9096351 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:9096351 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:9096351 | pubmed:author | pubmed-author:LieTT | lld:pubmed |
| pubmed-article:9096351 | pubmed:author | pubmed-author:ZhouHH | lld:pubmed |
| pubmed-article:9096351 | pubmed:author | pubmed-author:CostaR HRH | lld:pubmed |
| pubmed-article:9096351 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9096351 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:9096351 | pubmed:volume | 94 | lld:pubmed |
| pubmed-article:9096351 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9096351 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9096351 | pubmed:pagination | 3094-9 | lld:pubmed |
| pubmed-article:9096351 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:9096351 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9096351 | pubmed:articleTitle | The winged helix transcription factor HFH-4 is expressed during choroid plexus epithelial development in the mouse embryo. | lld:pubmed |
| pubmed-article:9096351 | pubmed:affiliation | University of Illinois at Chicago, College of Medicine, Department of Biochemistry, 60612-7334, USA. | lld:pubmed |
| pubmed-article:9096351 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9096351 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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