| pubmed-article:9096346 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9096346 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:9096346 | lifeskim:mentions | umls-concept:C0227525 | lld:lifeskim |
| pubmed-article:9096346 | lifeskim:mentions | umls-concept:C0025251 | lld:lifeskim |
| pubmed-article:9096346 | lifeskim:mentions | umls-concept:C0521451 | lld:lifeskim |
| pubmed-article:9096346 | lifeskim:mentions | umls-concept:C0085403 | lld:lifeskim |
| pubmed-article:9096346 | lifeskim:mentions | umls-concept:C0442805 | lld:lifeskim |
| pubmed-article:9096346 | lifeskim:mentions | umls-concept:C2700592 | lld:lifeskim |
| pubmed-article:9096346 | lifeskim:mentions | umls-concept:C0019409 | lld:lifeskim |
| pubmed-article:9096346 | lifeskim:mentions | umls-concept:C1265875 | lld:lifeskim |
| pubmed-article:9096346 | lifeskim:mentions | umls-concept:C0580836 | lld:lifeskim |
| pubmed-article:9096346 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:9096346 | pubmed:dateCreated | 1997-5-8 | lld:pubmed |
| pubmed-article:9096346 | pubmed:abstractText | Mitochondrial function during aging was assessed in isolated rat hepatocytes to avoid the problem of differential lysis when old, fragile mitochondria are isolated. Rhodamine 123, a fluorescent dye that accumulates in mitochondria on the basis of their membrane potential, was used as a probe to determine whether this key function is affected by aging. A marked fluorescent heterogeneity was observed in hepatocytes from old (20-28 months) but not young (3-5 months) rats, suggesting age-associated alterations in mitochondrial membrane potential, the driving force for ATP synthesis. Three distinct cell subpopulations were separated by centrifugal elutriation; each exhibited a unique rhodamine 123 fluorescence pattern, with the largest population from old rats having significantly lower fluorescence than that seen in young rats. This apparent age-associated alteration in mitochondrial membrane potential was confirmed by measurements with radioactive tetraphenylphosphonium bromide. Cells from young rats had a calculated membrane potential of -154 mV, in contrast to that of the three subpopulations from old rats of -70 mV (the largest population), -93 mV, and -154 mV. Production of oxidants was examined using 2',7'dichlorofluorescin, a dye that forms a fluorescent product upon oxidation. The largest cell subpopulation and a minor one from old animals produced significantly more oxidants than cells from young rats. To investigate the molecular cause(s) for the heterogeneity, we determined the levels of an age-associated mtDNA deletion. No significant differences were seen in the three subpopulations, indicating that the mitochondrial decay is due to other mutations, epigenetic changes, or both. | lld:pubmed |
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| pubmed-article:9096346 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9096346 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9096346 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9096346 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9096346 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:9096346 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:9096346 | pubmed:author | pubmed-author:BALLR CRC | lld:pubmed |
| pubmed-article:9096346 | pubmed:author | pubmed-author:ParkJ YJY | lld:pubmed |
| pubmed-article:9096346 | pubmed:author | pubmed-author:BartholomewJ... | lld:pubmed |
| pubmed-article:9096346 | pubmed:author | pubmed-author:WehrC MCM | lld:pubmed |
| pubmed-article:9096346 | pubmed:author | pubmed-author:HagenT MTM | lld:pubmed |
| pubmed-article:9096346 | pubmed:author | pubmed-author:DeK RKR | lld:pubmed |
| pubmed-article:9096346 | pubmed:author | pubmed-author:YoweD LDL | lld:pubmed |
| pubmed-article:9096346 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9096346 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:9096346 | pubmed:volume | 94 | lld:pubmed |
| pubmed-article:9096346 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9096346 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9096346 | pubmed:pagination | 3064-9 | lld:pubmed |
| pubmed-article:9096346 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:9096346 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9096346 | pubmed:articleTitle | Mitochondrial decay in hepatocytes from old rats: membrane potential declines, heterogeneity and oxidants increase. | lld:pubmed |
| pubmed-article:9096346 | pubmed:affiliation | Division of Biochemistry and Molecular Biology, University of California, Berkeley 94720, USA. | lld:pubmed |
| pubmed-article:9096346 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9096346 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9096346 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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