| pubmed-article:9070882 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9070882 | lifeskim:mentions | umls-concept:C0005528 | lld:lifeskim |
| pubmed-article:9070882 | lifeskim:mentions | umls-concept:C0598849 | lld:lifeskim |
| pubmed-article:9070882 | lifeskim:mentions | umls-concept:C0007952 | lld:lifeskim |
| pubmed-article:9070882 | lifeskim:mentions | umls-concept:C0136082 | lld:lifeskim |
| pubmed-article:9070882 | lifeskim:mentions | umls-concept:C0597484 | lld:lifeskim |
| pubmed-article:9070882 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:9070882 | pubmed:dateCreated | 1997-4-16 | lld:pubmed |
| pubmed-article:9070882 | pubmed:abstractText | The electrophysiological characteristics of the Na+/I- symporter were examined using the Chinese hamster ovary (CHO) cell line, which was transfected with the rat Na+/I- symporter gene and stably expressed the Na+/I- symporter. In this cell line, iodide uptake was dependent on Na+, and kinetic studies revealed that the K(m) for iodide was 35 microM, similar to that of FRTL-5 cells. The maximal velocity at the cell protein level was 6- to 10-fold higher than in FRTL-5 cells. ClO-4 and SCN- dose-dependently inhibited iodide uptake in a competitive manner. Electrophysiological characteristics were examined using the whole-cell patch-clamp technique. The holding current at-40 mV rapidly shifted inwardly when the cells were perfused with 1 mEq I- or SCN-. The inward current induced by 1 mEq I- did not increase when bathing solution was replaced with a Tyrode solution with 10 mEq I-, indicating that 1 mEq I- was a saturating amount. The inward current induced by 1 mEq I- increased 1.5-fold by changing the bathing solution to a Tyrode solution containing 1 mEq I- and 1 mEq SCN-. The inward current induced by 0.5 mEq SCN- decreased when the bathing solution was changed to a Tyrode solution containing 0.5 mEq SCN- and 10 mEq I-. These findings indicated that the I- ion and the SCN- ion were carried by the NA+/ I- symporter with at least two Na+ ions. The current induced by the transport of SCN- was larger than that induced by the transport of I-, possibly because the number of Na+ ions that was carried with one SCN- ion was larger than the number of Na+ ions carried with one I- ion. Surprisingly, the perfusion of ClO-4 did not induce an inward current, indicating that ClO-4 bound to the Na+/I- symporter, but was not carried by it, or that one ClO-4 ion was carried with one Na+ ion. | lld:pubmed |
| pubmed-article:9070882 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9070882 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9070882 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9070882 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9070882 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9070882 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9070882 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9070882 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9070882 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9070882 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9070882 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9070882 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:9070882 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:9070882 | pubmed:author | pubmed-author:YoshidaAA | lld:pubmed |
| pubmed-article:9070882 | pubmed:author | pubmed-author:MoriAA | lld:pubmed |
| pubmed-article:9070882 | pubmed:author | pubmed-author:SatoRR | lld:pubmed |
| pubmed-article:9070882 | pubmed:author | pubmed-author:MoriTT | lld:pubmed |
| pubmed-article:9070882 | pubmed:author | pubmed-author:HattoriKK | lld:pubmed |
| pubmed-article:9070882 | pubmed:author | pubmed-author:HisatomiMM | lld:pubmed |
| pubmed-article:9070882 | pubmed:author | pubmed-author:MitaniYY | lld:pubmed |
| pubmed-article:9070882 | pubmed:author | pubmed-author:SasakiNN | lld:pubmed |
| pubmed-article:9070882 | pubmed:author | pubmed-author:TaniguchiSS | lld:pubmed |
| pubmed-article:9070882 | pubmed:author | pubmed-author:UetaYY | lld:pubmed |
| pubmed-article:9070882 | pubmed:author | pubmed-author:ShigemasaCC | lld:pubmed |
| pubmed-article:9070882 | pubmed:author | pubmed-author:KosugiSS | lld:pubmed |
| pubmed-article:9070882 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9070882 | pubmed:day | 24 | lld:pubmed |
| pubmed-article:9070882 | pubmed:volume | 231 | lld:pubmed |
| pubmed-article:9070882 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9070882 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9070882 | pubmed:pagination | 731-4 | lld:pubmed |
| pubmed-article:9070882 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9070882 | pubmed:meshHeading | pubmed-meshheading:9070882-... | lld:pubmed |
| pubmed-article:9070882 | pubmed:meshHeading | pubmed-meshheading:9070882-... | lld:pubmed |
| pubmed-article:9070882 | pubmed:meshHeading | pubmed-meshheading:9070882-... | lld:pubmed |
| pubmed-article:9070882 | pubmed:meshHeading | pubmed-meshheading:9070882-... | lld:pubmed |
| pubmed-article:9070882 | pubmed:meshHeading | pubmed-meshheading:9070882-... | lld:pubmed |
| pubmed-article:9070882 | pubmed:meshHeading | pubmed-meshheading:9070882-... | lld:pubmed |
| pubmed-article:9070882 | pubmed:meshHeading | pubmed-meshheading:9070882-... | lld:pubmed |
| pubmed-article:9070882 | pubmed:meshHeading | pubmed-meshheading:9070882-... | lld:pubmed |
| pubmed-article:9070882 | pubmed:meshHeading | pubmed-meshheading:9070882-... | lld:pubmed |
| pubmed-article:9070882 | pubmed:meshHeading | pubmed-meshheading:9070882-... | lld:pubmed |
| pubmed-article:9070882 | pubmed:meshHeading | pubmed-meshheading:9070882-... | lld:pubmed |
| pubmed-article:9070882 | pubmed:meshHeading | pubmed-meshheading:9070882-... | lld:pubmed |
| pubmed-article:9070882 | pubmed:meshHeading | pubmed-meshheading:9070882-... | lld:pubmed |
| pubmed-article:9070882 | pubmed:meshHeading | pubmed-meshheading:9070882-... | lld:pubmed |
| pubmed-article:9070882 | pubmed:meshHeading | pubmed-meshheading:9070882-... | lld:pubmed |
| pubmed-article:9070882 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9070882 | pubmed:articleTitle | Different electrophysiological character of I-, ClO4-, and SCN- in the transport by Na+/I- symporter. | lld:pubmed |
| pubmed-article:9070882 | pubmed:affiliation | First Department of Internal Medicine, Tottori University School of Medicine, Yonago, Japan. | lld:pubmed |
| pubmed-article:9070882 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9070882 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9070882 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9070882 | lld:pubmed |
