| pubmed-article:9069455 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9069455 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9069455 | lifeskim:mentions | umls-concept:C0085413 | lld:lifeskim |
| pubmed-article:9069455 | lifeskim:mentions | umls-concept:C0599635 | lld:lifeskim |
| pubmed-article:9069455 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:9069455 | lifeskim:mentions | umls-concept:C0475264 | lld:lifeskim |
| pubmed-article:9069455 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:9069455 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:9069455 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:9069455 | pubmed:dateCreated | 1997-7-15 | lld:pubmed |
| pubmed-article:9069455 | pubmed:abstractText | To characterize the cyst-lining cells in human autosomal dominant polycystic kidney disease (ADPKD), we performed immunohistological studies with specific antibodies against human aquaporin-2 (AQP-2, the vasopressin-regulated water channel) and aquaporin-3 (AQP-3), which are expressed only in collecting duct cells in the normal kidney. The polycystic kidney samples were obtained from 2 hemodialysis patient at uninephrectomy. Immunohistochemical studies revealed two types of staining of cyst-lining cells. Approximately 30% of all the cysts were simultaneously immunostained by both antibodies. Among these AQP-positive cysts, more than 90% of the cysts were intensely stained, with well-polarized localization of AQP-2 and AQP-3. In fewer than 10% of AQP-positive cysts, by contrast, immunostaining for AQP-2 and AQP-3 was faint and no clearly polarized localization of the channels was observed. We examined the immunostaining in further detail by electron microscopy. Staining specific for AQP-2 was mainly observed in the apical membrane of cyst-lining cells. Moreover, staining specific for AQP-3 was observed in all of the AQP-2-positive cysts. It appeared unlikely that the variations in immunostaining observed under the light microscope had been induced by total disruption of water-channel polarity. The present study suggests that about 30% of the cysts in our cases of ADPKD were derived from the collecting duct cells and that the cyst-lining cells were well differentiated in terms of AQP expression. | lld:pubmed |
| pubmed-article:9069455 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9069455 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9069455 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9069455 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9069455 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9069455 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9069455 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9069455 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9069455 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9069455 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9069455 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9069455 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9069455 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9069455 | pubmed:issn | 0028-2766 | lld:pubmed |
| pubmed-article:9069455 | pubmed:author | pubmed-author:HayashiMM | lld:pubmed |
| pubmed-article:9069455 | pubmed:author | pubmed-author:SasakiSS | lld:pubmed |
| pubmed-article:9069455 | pubmed:author | pubmed-author:YoshidaTT | lld:pubmed |
| pubmed-article:9069455 | pubmed:author | pubmed-author:IshibashiKK | lld:pubmed |
| pubmed-article:9069455 | pubmed:author | pubmed-author:SarutaTT | lld:pubmed |
| pubmed-article:9069455 | pubmed:author | pubmed-author:KitajimaWW | lld:pubmed |
| pubmed-article:9069455 | pubmed:author | pubmed-author:YamajiYY | lld:pubmed |
| pubmed-article:9069455 | pubmed:author | pubmed-author:SasamuraHH | lld:pubmed |
| pubmed-article:9069455 | pubmed:author | pubmed-author:MonkawaTT | lld:pubmed |
| pubmed-article:9069455 | pubmed:author | pubmed-author:TsuganezawaHH | lld:pubmed |
| pubmed-article:9069455 | pubmed:author | pubmed-author:MaurmoFF | lld:pubmed |
| pubmed-article:9069455 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9069455 | pubmed:volume | 75 | lld:pubmed |
| pubmed-article:9069455 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9069455 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9069455 | pubmed:pagination | 321-6 | lld:pubmed |
| pubmed-article:9069455 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9069455 | pubmed:meshHeading | pubmed-meshheading:9069455-... | lld:pubmed |
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| pubmed-article:9069455 | pubmed:meshHeading | pubmed-meshheading:9069455-... | lld:pubmed |
| pubmed-article:9069455 | pubmed:meshHeading | pubmed-meshheading:9069455-... | lld:pubmed |
| pubmed-article:9069455 | pubmed:meshHeading | pubmed-meshheading:9069455-... | lld:pubmed |
| pubmed-article:9069455 | pubmed:meshHeading | pubmed-meshheading:9069455-... | lld:pubmed |
| pubmed-article:9069455 | pubmed:meshHeading | pubmed-meshheading:9069455-... | lld:pubmed |
| pubmed-article:9069455 | pubmed:meshHeading | pubmed-meshheading:9069455-... | lld:pubmed |
| pubmed-article:9069455 | pubmed:meshHeading | pubmed-meshheading:9069455-... | lld:pubmed |
| pubmed-article:9069455 | pubmed:meshHeading | pubmed-meshheading:9069455-... | lld:pubmed |
| pubmed-article:9069455 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9069455 | pubmed:articleTitle | Expression and localization of the water channels in human autosomal dominant polycystic kidney disease. | lld:pubmed |
| pubmed-article:9069455 | pubmed:affiliation | Department of Internal Medicine, Keio University, School of Medicine, Shinjuku-ku, Tokyo, Japan. | lld:pubmed |
| pubmed-article:9069455 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9069455 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9069455 | lld:pubmed |
