| pubmed-article:9022284 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9022284 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:9022284 | lifeskim:mentions | umls-concept:C1135183 | lld:lifeskim |
| pubmed-article:9022284 | lifeskim:mentions | umls-concept:C0013935 | lld:lifeskim |
| pubmed-article:9022284 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:9022284 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:9022284 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:9022284 | lifeskim:mentions | umls-concept:C0332255 | lld:lifeskim |
| pubmed-article:9022284 | lifeskim:mentions | umls-concept:C0178499 | lld:lifeskim |
| pubmed-article:9022284 | lifeskim:mentions | umls-concept:C1527178 | lld:lifeskim |
| pubmed-article:9022284 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:9022284 | pubmed:dateCreated | 1997-3-28 | lld:pubmed |
| pubmed-article:9022284 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9022284 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9022284 | pubmed:abstractText | Between days 11 and 12 of gestation, the porcine conceptus undergoes a metamorphosis from a spherical blastocyst to an elongate thread-like form. During this process, the conceptus secretes a variety of products. One of these products is protein previously referred to as porcine embryonic basic protein (BP). This protein has been shown to be a major secreted product between days 13 and 18. In this study, we report a simple two-step procedure to isolate BP from day 15 porcine conceptus conditioned medium, utilized ion-exchange chromatography and reverse-phase HPLC. Purified BP was subjected to Edman degradation amino-terminal sequencing and a 25 amino acid residue sequence was obtained. Comparing the N-terminal sequence of BP to sequences in the GenBank database determined that BP shared amino acid homology with porcine pregnancy-associated glycoprotein-2 (PAG-2). The region of identity corresponded to an internal site of PAG-2, suggesting BP was a proteolytic fragment of PAG-2. The purified protein was confirmed to be BP by Western blot using a previously characterized anti-BP antiserum. Also, the BP was immunolocalized with the trophectoderm of day 11 blastocysts. Staining intensity was diminished in spherical blastocysts compared to elongated blastocysts. Although the function of PAG-2 and its cleavage product BP are unknown, the large quantity produced by the porcine conceptus and its sequence conservation across species may indicate a necessary role in early pregnancy. | lld:pubmed |
| pubmed-article:9022284 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9022284 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9022284 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9022284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9022284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9022284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9022284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9022284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9022284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9022284 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9022284 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:9022284 | pubmed:issn | 1357-2725 | lld:pubmed |
| pubmed-article:9022284 | pubmed:author | pubmed-author:GodkinJ DJD | lld:pubmed |
| pubmed-article:9022284 | pubmed:author | pubmed-author:BíroVV | lld:pubmed |
| pubmed-article:9022284 | pubmed:author | pubmed-author:KatteshH GHG | lld:pubmed |
| pubmed-article:9022284 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9022284 | pubmed:volume | 28 | lld:pubmed |
| pubmed-article:9022284 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9022284 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9022284 | pubmed:pagination | 1249-55 | lld:pubmed |
| pubmed-article:9022284 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:9022284 | pubmed:meshHeading | pubmed-meshheading:9022284-... | lld:pubmed |
| pubmed-article:9022284 | pubmed:meshHeading | pubmed-meshheading:9022284-... | lld:pubmed |
| pubmed-article:9022284 | pubmed:meshHeading | pubmed-meshheading:9022284-... | lld:pubmed |
| pubmed-article:9022284 | pubmed:meshHeading | pubmed-meshheading:9022284-... | lld:pubmed |
| pubmed-article:9022284 | pubmed:meshHeading | pubmed-meshheading:9022284-... | lld:pubmed |
| pubmed-article:9022284 | pubmed:meshHeading | pubmed-meshheading:9022284-... | lld:pubmed |
| pubmed-article:9022284 | pubmed:meshHeading | pubmed-meshheading:9022284-... | lld:pubmed |
| pubmed-article:9022284 | pubmed:meshHeading | pubmed-meshheading:9022284-... | lld:pubmed |
| pubmed-article:9022284 | pubmed:meshHeading | pubmed-meshheading:9022284-... | lld:pubmed |
| pubmed-article:9022284 | pubmed:meshHeading | pubmed-meshheading:9022284-... | lld:pubmed |
| pubmed-article:9022284 | pubmed:meshHeading | pubmed-meshheading:9022284-... | lld:pubmed |
| pubmed-article:9022284 | pubmed:meshHeading | pubmed-meshheading:9022284-... | lld:pubmed |
| pubmed-article:9022284 | pubmed:meshHeading | pubmed-meshheading:9022284-... | lld:pubmed |
| pubmed-article:9022284 | pubmed:meshHeading | pubmed-meshheading:9022284-... | lld:pubmed |
| pubmed-article:9022284 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:9022284 | pubmed:articleTitle | Isolation and identification of porcine embryonic basic protein as a fragment of pregnancy-associated glycoprotein-2. | lld:pubmed |
| pubmed-article:9022284 | pubmed:affiliation | Department of Animal Science, University of Tennessee, Knoxville 37901, USA. | lld:pubmed |
| pubmed-article:9022284 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9022284 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
