| pubmed-article:9021928 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9021928 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
| pubmed-article:9021928 | lifeskim:mentions | umls-concept:C0006669 | lld:lifeskim |
| pubmed-article:9021928 | lifeskim:mentions | umls-concept:C0079189 | lld:lifeskim |
| pubmed-article:9021928 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:9021928 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:9021928 | lifeskim:mentions | umls-concept:C0205132 | lld:lifeskim |
| pubmed-article:9021928 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:9021928 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:9021928 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:9021928 | pubmed:dateCreated | 1997-2-26 | lld:pubmed |
| pubmed-article:9021928 | pubmed:abstractText | Calcitonin gene-related peptide (CGRP) inhibits antigen presentation by Langerhans cells (LC) and macrophages, and LC are anatomically associated with CGRP-containing epidermal nerves. To determine whether CGRP may produce some of its functional effects through regulation of cytokine expression, we utilized enzyme-linked immunosorbent assay (ELISA) of conditioned supernatants to examine production of interleukin (IL)-10 and IL-1 beta protein in the LC-like cell line XS52 as well as the reverse transcriptase-polymerase chain reaction (RT-PCR) to examine levels of mRNA for IL-10, IL-1 beta, and the 40-kDa subunit (p40) of IL-12. CGRP augmented the lipopolysaccharide (LPS) and granulocyte-macrophage colony-stimulating factor (GM-CSF) -induced release of IL-10 protein and the induced expression of IL-10 mRNA in these cells. However, it suppressed the induction of release of IL-1 beta protein and the induction of mRNA for IL-12 p40 and IL-1 beta by LPS and GM-CSF. Regulation of cytokine expression in peritoneal macrophages was also examined. By ELISA, the LPS-induced expression of IL-10 was augmented by CGRP, whereas the induction of IL-1 beta was suppressed. Northern analysis demonstrated augmentation of LPS-induced IL-10 mRNA levels and inhibition of LPS-induced IL-1 beta mRNA by CGRP. CGRP inhibited the LPS-induced induction of IL-12 mRNA as assessed by RT-PCR. Up-regulation of B7-2 expression by LPS and GM-CSF was suppressed by CGRP in both XS52 cells and macrophages, as previously reported. This suppression, however, could be abrogated by co-culture with neutralizing antibodies to IL-10. Furthermore, the presence of neutralizing antibodies to IL-10 during exposure of epidermal cells (EC) to CGRP prevented the CGRP-mediated suppression of EC presentation of tumor-associated antigens (from the S1509a spindle cell carcinoma) for elicitation of delayed-type hypersensitivity in S1509a-immune mice. These data suggest that suppression of antigen-presenting function by CGRP is mediated, at least in part, by changes in cytokine expression that favor less robust antigen presentation for cell-mediated immunity. | lld:pubmed |
| pubmed-article:9021928 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9021928 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9021928 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9021928 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9021928 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9021928 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9021928 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9021928 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9021928 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9021928 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9021928 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9021928 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9021928 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9021928 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9021928 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9021928 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9021928 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:9021928 | pubmed:issn | 0741-5400 | lld:pubmed |
| pubmed-article:9021928 | pubmed:author | pubmed-author:ToriiHH | lld:pubmed |
| pubmed-article:9021928 | pubmed:author | pubmed-author:RushE CEC | lld:pubmed |
| pubmed-article:9021928 | pubmed:author | pubmed-author:HosoiJJ | lld:pubmed |
| pubmed-article:9021928 | pubmed:author | pubmed-author:TakashimaAA | lld:pubmed |
| pubmed-article:9021928 | pubmed:author | pubmed-author:GAYJ GJG | lld:pubmed |
| pubmed-article:9021928 | pubmed:author | pubmed-author:FoxF EFE | lld:pubmed |
| pubmed-article:9021928 | pubmed:author | pubmed-author:GransteinR... | lld:pubmed |
| pubmed-article:9021928 | pubmed:author | pubmed-author:BeissertSS | lld:pubmed |
| pubmed-article:9021928 | pubmed:author | pubmed-author:AsahinaAA | lld:pubmed |
| pubmed-article:9021928 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9021928 | pubmed:volume | 61 | lld:pubmed |
| pubmed-article:9021928 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9021928 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9021928 | pubmed:pagination | 216-23 | lld:pubmed |
| pubmed-article:9021928 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:9021928 | pubmed:meshHeading | pubmed-meshheading:9021928-... | lld:pubmed |
| pubmed-article:9021928 | pubmed:meshHeading | pubmed-meshheading:9021928-... | lld:pubmed |
| pubmed-article:9021928 | pubmed:meshHeading | pubmed-meshheading:9021928-... | lld:pubmed |
| pubmed-article:9021928 | pubmed:meshHeading | pubmed-meshheading:9021928-... | lld:pubmed |
| pubmed-article:9021928 | pubmed:meshHeading | pubmed-meshheading:9021928-... | lld:pubmed |
| pubmed-article:9021928 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9021928 | pubmed:articleTitle | Regulation of cytokine expression in macrophages and the Langerhans cell-like line XS52 by calcitonin gene-related peptide. | lld:pubmed |
| pubmed-article:9021928 | pubmed:affiliation | Massachusetts General Hospital/Harvard Cutaneous Biology Research Center, Boston, USA. | lld:pubmed |
| pubmed-article:9021928 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9021928 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9021928 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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