| pubmed-article:9006968 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9006968 | lifeskim:mentions | umls-concept:C1882598 | lld:lifeskim |
| pubmed-article:9006968 | lifeskim:mentions | umls-concept:C1172779 | lld:lifeskim |
| pubmed-article:9006968 | lifeskim:mentions | umls-concept:C2745955 | lld:lifeskim |
| pubmed-article:9006968 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:9006968 | lifeskim:mentions | umls-concept:C1709634 | lld:lifeskim |
| pubmed-article:9006968 | lifeskim:mentions | umls-concept:C0667999 | lld:lifeskim |
| pubmed-article:9006968 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:9006968 | pubmed:dateCreated | 1997-3-10 | lld:pubmed |
| pubmed-article:9006968 | pubmed:abstractText | It has been suggested that anandamide (N-arachidonoylethanolamine), an endogenous cannabinoid substance, may be produced through Ca2+-stimulated hydrolysis of the phosphatidylethanolamine (PE) derivative N-arachidonoyl PE. The presence of N-arachidonoyl PE in adult brain tissue and the enzyme pathways that underlie its biosynthesis are, however, still undetermined. We report here that rat brain tissue contains both anandamide (11 +/- 7 pmol/gm wet tissue) and N-arachidonoyl PE (22 +/- 16 pmol/gm), as assessed by gas chromatography/mass spectrometry. We describe a N-acyltransferase activity in brain that catalyzes the biosynthesis of N-arachidonoyl PE by transferring an arachidonate group from the sn-1 carbon of phospholipids to the amino group of PE. We also show that sn-1 arachidonoyl phospholipids are present in brain, where they constitute approximately 0.5% of total phospholipids. N-acyltransferase activity is Ca2+ dependent and is enriched in brain and testis. Within the brain, N-acyltransferase activity is highest in brainstem; intermediate in cortex, striatum, hippocampus, medulla, and cerebellum; and lowest in thalamus, hypothalamus, and olfactory bulb. Pharmacological inhibition of N-acyltransferase activity in primary cultures of cortical neurons prevents Ca2+-stimulated N-arachidonoyl PE biosynthesis. Our results demonstrate, therefore, that rat brain tissue contains the complement of enzymatic activity and lipid substrates necessary for the biosynthesis of the anandamide precursor N-arachidonoyl PE. They also suggest that biosynthesis of N-arachidonoyl PE and formation of anandamide are tightly coupled processes, which may concomitantly be stimulated by elevations in intracellular Ca2+ occurring during neural activity. | lld:pubmed |
| pubmed-article:9006968 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9006968 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9006968 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9006968 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9006968 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:9006968 | pubmed:issn | 0270-6474 | lld:pubmed |
| pubmed-article:9006968 | pubmed:author | pubmed-author:PiomelliDD | lld:pubmed |
| pubmed-article:9006968 | pubmed:author | pubmed-author:CadasHH | lld:pubmed |
| pubmed-article:9006968 | pubmed:author | pubmed-author:di TomasoEE | lld:pubmed |
| pubmed-article:9006968 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9006968 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:9006968 | pubmed:volume | 17 | lld:pubmed |
| pubmed-article:9006968 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9006968 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9006968 | pubmed:pagination | 1226-42 | lld:pubmed |
| pubmed-article:9006968 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:9006968 | pubmed:meshHeading | pubmed-meshheading:9006968-... | lld:pubmed |
| pubmed-article:9006968 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9006968 | pubmed:articleTitle | Occurrence and biosynthesis of endogenous cannabinoid precursor, N-arachidonoyl phosphatidylethanolamine, in rat brain. | lld:pubmed |
| pubmed-article:9006968 | pubmed:affiliation | The Neurosciences Institute, San Diego, California 92121, USA. | lld:pubmed |
| pubmed-article:9006968 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9006968 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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