| pubmed-article:8991542 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8991542 | lifeskim:mentions | umls-concept:C0021760 | lld:lifeskim |
| pubmed-article:8991542 | lifeskim:mentions | umls-concept:C0018951 | lld:lifeskim |
| pubmed-article:8991542 | lifeskim:mentions | umls-concept:C0011306 | lld:lifeskim |
| pubmed-article:8991542 | lifeskim:mentions | umls-concept:C0079189 | lld:lifeskim |
| pubmed-article:8991542 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8991542 | pubmed:dateCreated | 1997-1-15 | lld:pubmed |
| pubmed-article:8991542 | pubmed:abstractText | The aim of this study was to investigate the role of interleukin 6 (IL-6) in normal dendritic cell (DC) hematopoiesis. We used an enzyme-linked immunosorbent assay to quantitate IL-6 levels in CD34+ progenitor cell cultures favoring monocyte (mono) development versus those supporting mono-DC growth, and studied the neutralizing effects of alphaIL-6 antibody on DC hematopoiesis. IL6 levels in mono cultures (GM-CSF alone) were detected by day 4 and remained constant (approximately 100+ pg/ml) for 18 days. In mono-DC cultures, higher IL-6 levels correlated with DC content and development. Short-term mono-DC cultures initiated with GM-CSF + tumor necrosis factor (TNF) + stem cell factor (SCF) exhibited increases in IL-6 level until day 11 (peak DC growth). By day 18, the levels had declined and cells expressing typical DC features were no longer present. Long-term mono-DC cultures sustained with GM-CSF + TNF + SCF contained the highest IL-6 levels (671 pg/ml) on day 11. In these cultures, DCs and higher IL-6 levels persisted beyond 18 days. Anti-IL-6 profoundly inhibited cell proliferation associated with DC hematopoiesis when added on days 0, 2 and 5 to GM-CSF + TNF + SCF cultures, indicating that various stages of mono-DC development rely on IL-6. There was no reduction in the T cell response when alphaIL-6 was added to mixed leukocyte reaction cultures containing mature DCs as stimulators. Thus, alphaIL-6 appears to downregulate developmental processes associated with optimal mono-DC growth, but not the effector functions of mature DCs. These studies substantiate the importance of IL-6 as a secondary cytokine during DC development and provide insight into another control point in the DC pathway. | lld:pubmed |
| pubmed-article:8991542 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8991542 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8991542 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8991542 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8991542 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8991542 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8991542 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:8991542 | pubmed:issn | 1066-5099 | lld:pubmed |
| pubmed-article:8991542 | pubmed:author | pubmed-author:Santiago-Schw... | lld:pubmed |
| pubmed-article:8991542 | pubmed:author | pubmed-author:CarsonsS ESE | lld:pubmed |
| pubmed-article:8991542 | pubmed:author | pubmed-author:TucciJJ | lld:pubmed |
| pubmed-article:8991542 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8991542 | pubmed:volume | 14 | lld:pubmed |
| pubmed-article:8991542 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8991542 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8991542 | pubmed:pagination | 225-31 | lld:pubmed |
| pubmed-article:8991542 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:8991542 | pubmed:meshHeading | pubmed-meshheading:8991542-... | lld:pubmed |
| pubmed-article:8991542 | pubmed:meshHeading | pubmed-meshheading:8991542-... | lld:pubmed |
| pubmed-article:8991542 | pubmed:meshHeading | pubmed-meshheading:8991542-... | lld:pubmed |
| pubmed-article:8991542 | pubmed:meshHeading | pubmed-meshheading:8991542-... | lld:pubmed |
| pubmed-article:8991542 | pubmed:meshHeading | pubmed-meshheading:8991542-... | lld:pubmed |
| pubmed-article:8991542 | pubmed:meshHeading | pubmed-meshheading:8991542-... | lld:pubmed |
| pubmed-article:8991542 | pubmed:meshHeading | pubmed-meshheading:8991542-... | lld:pubmed |
| pubmed-article:8991542 | pubmed:meshHeading | pubmed-meshheading:8991542-... | lld:pubmed |
| pubmed-article:8991542 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8991542 | pubmed:articleTitle | Endogenously produced interleukin 6 is an accessory cytokine for dendritic cell hematopoiesis. | lld:pubmed |
| pubmed-article:8991542 | pubmed:affiliation | Division of Rheumatology, Allergy & Immunology, Winthrop University Hospital, Mineola, New York 11501, USA. | lld:pubmed |
| pubmed-article:8991542 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8991542 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8991542 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8991542 | lld:pubmed |
