| pubmed-article:8978594 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8978594 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:8978594 | lifeskim:mentions | umls-concept:C1383501 | lld:lifeskim |
| pubmed-article:8978594 | lifeskim:mentions | umls-concept:C0086045 | lld:lifeskim |
| pubmed-article:8978594 | lifeskim:mentions | umls-concept:C0007587 | lld:lifeskim |
| pubmed-article:8978594 | lifeskim:mentions | umls-concept:C0333516 | lld:lifeskim |
| pubmed-article:8978594 | lifeskim:mentions | umls-concept:C0442805 | lld:lifeskim |
| pubmed-article:8978594 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:8978594 | lifeskim:mentions | umls-concept:C0439834 | lld:lifeskim |
| pubmed-article:8978594 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8978594 | pubmed:dateCreated | 1997-1-16 | lld:pubmed |
| pubmed-article:8978594 | pubmed:abstractText | The potential involvement of cellular calcium in the signalling pathway of tumour necrosis factor-alpha (TNF) was assessed in L929 cells using 45Ca2+ and confocal laser scanning microscopy with fluorescence calcium indicators. Our data indicate that the effect of TNF on intracellular Ca2+ mobilization is a slow process with no discernible increase in the cytosolic free Ca2+ concentration ([Ca2+]c) and intranuclear Ca2+ level ([Ca2+]n) within the 1st min of TNF (25 ng/ml) administration. However, prolonged exposure (2 h) of L929 cells to TNF brought about pronounced increase in cytosolic and intranuclear [Ca2+] even in the absence of external Ca2+. The increase in intracellular [Ca2+] was more apparent when cells were treated with thapsigargin, an inhibitor of microsomal Ca2+-ATPase. Interestingly, most of the Ca2+ released was around and confined to the nucleus. Following the pretreatment of cells with thapsigargin, a synergistic killing effect was obtained when cells were cultured with TNF. The use of 45Ca2+ also revealed that TNF enhanced the 45Ca2+ uptake in a time-dependent manner. Calcium channel blockers, verapamil and diltiazem, could alleviate both the TNF-mediated 45Ca2+-uptake and killing activity. Our results therefore suggest that an increase in cellular Ca2+ is a crucial factor in the TNF cytotoxicity. | lld:pubmed |
| pubmed-article:8978594 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8978594 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8978594 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8978594 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8978594 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8978594 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8978594 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8978594 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8978594 | pubmed:issn | 0030-2414 | lld:pubmed |
| pubmed-article:8978594 | pubmed:author | pubmed-author:FruhE GEG | lld:pubmed |
| pubmed-article:8978594 | pubmed:author | pubmed-author:LeeC YCY | lld:pubmed |
| pubmed-article:8978594 | pubmed:author | pubmed-author:ChoyY MYM | lld:pubmed |
| pubmed-article:8978594 | pubmed:author | pubmed-author:KongS KSK | lld:pubmed |
| pubmed-article:8978594 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8978594 | pubmed:volume | 54 | lld:pubmed |
| pubmed-article:8978594 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8978594 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8978594 | pubmed:pagination | 55-62 | lld:pubmed |
| pubmed-article:8978594 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8978594 | pubmed:meshHeading | pubmed-meshheading:8978594-... | lld:pubmed |
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| pubmed-article:8978594 | pubmed:meshHeading | pubmed-meshheading:8978594-... | lld:pubmed |
| pubmed-article:8978594 | pubmed:meshHeading | pubmed-meshheading:8978594-... | lld:pubmed |
| pubmed-article:8978594 | pubmed:articleTitle | Slow increase in intranuclear and cytosolic free calcium concentrations in L929 cells is important in tumour necrosis factor-alpha-mediated cell death. | lld:pubmed |
| pubmed-article:8978594 | pubmed:affiliation | Department of Biochemistry, Chinese University of Hong Kong, Shatin. | lld:pubmed |
| pubmed-article:8978594 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8978594 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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