| pubmed-article:8973561 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8973561 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:8973561 | lifeskim:mentions | umls-concept:C0036003 | lld:lifeskim |
| pubmed-article:8973561 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:8973561 | lifeskim:mentions | umls-concept:C0029000 | lld:lifeskim |
| pubmed-article:8973561 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:8973561 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:8973561 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:8973561 | pubmed:dateCreated | 1997-1-27 | lld:pubmed |
| pubmed-article:8973561 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8973561 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8973561 | pubmed:abstractText | Complete cDNA and genomic sequences encoding the Onchocerca volvulus S-adenosylmethionine decarboxylase (SAMDC), a key enzyme in polyamine biosynthesis, have been isolated and characterized. The deduced amino acid sequence encodes a 42 kDa proenzyme with a moderate level of sequence homology to eukaryotic SAMDCs. Enzymically active O. volvulus SAMDC was expressed at a high level in an Escherichia coli mutant strain lacking endogenous SAMDC. The recombinant enzyme was purified to homogeneity using DEAE-cellulose, methylglyoxal bis(guanylhydrazone)-Sepharose and Superdex S-200 chromatography. It was determined that the recombinant proenzyme is cleaved to produce 32 and 10 kDa subunits. The sequence of the N-terminal portion of the large subunit was determined and comparison with the sequence of the proenzyme revealed that the precise cleavage site lies between Glu86 and Ser87. Gel-filtration experiments demonstrated that these two subunits combine to form an active heterotetramer. Comparison of the cDNA and genomic sequences revealed that the SAMDC mRNA undergoes both cis- and trans-splicing in its 5'-untranslated region (UTR). Anchored PCR on O. volvulus mRNA confirmed the cDNA sequence and identified two distinct trans-spliced products, a 22-nucleotide spliced-leader sequence and a 138 bp sequence containing the 22 nucleotide spliced-leader sequence. Genomic Southern-blot analysis suggests that the O. volvulus SAMDC is encoded by a single-copy gene. This gene spans 5.3 kb and is comprised of nine exons and eight introns. The first intron is located in the 5'-UTR and processing of this intron has a potential regulatory function. The 5'-flanking region of the gene contains potential transcriptional regulatory elements such as a TATA box, two CAAT boxes and AP-1-, C/EBP-, ELP-, H-APF-1-, HNF-5- and PEA3-binding sites. | lld:pubmed |
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| pubmed-article:8973561 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8973561 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8973561 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8973561 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8973561 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8973561 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:8973561 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:8973561 | pubmed:author | pubmed-author:WalterR DRD | lld:pubmed |
| pubmed-article:8973561 | pubmed:author | pubmed-author:Henkle-Dührse... | lld:pubmed |
| pubmed-article:8973561 | pubmed:author | pubmed-author:Da'DaraA AAA | lld:pubmed |
| pubmed-article:8973561 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8973561 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:8973561 | pubmed:volume | 320 ( Pt 2) | lld:pubmed |
| pubmed-article:8973561 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8973561 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8973561 | pubmed:pagination | 519-30 | lld:pubmed |
| pubmed-article:8973561 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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