| pubmed-article:8958135 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8958135 | lifeskim:mentions | umls-concept:C0521119 | lld:lifeskim |
| pubmed-article:8958135 | lifeskim:mentions | umls-concept:C0151744 | lld:lifeskim |
| pubmed-article:8958135 | lifeskim:mentions | umls-concept:C0030054 | lld:lifeskim |
| pubmed-article:8958135 | lifeskim:mentions | umls-concept:C0523807 | lld:lifeskim |
| pubmed-article:8958135 | lifeskim:mentions | umls-concept:C1705425 | lld:lifeskim |
| pubmed-article:8958135 | lifeskim:mentions | umls-concept:C0181496 | lld:lifeskim |
| pubmed-article:8958135 | lifeskim:mentions | umls-concept:C1516048 | lld:lifeskim |
| pubmed-article:8958135 | lifeskim:mentions | umls-concept:C1522485 | lld:lifeskim |
| pubmed-article:8958135 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:8958135 | pubmed:dateCreated | 1997-4-10 | lld:pubmed |
| pubmed-article:8958135 | pubmed:abstractText | We describe real-time measurement of myocardial oxygen consumption during ischemia in the intact heart. Measurement of extracellular oxygen concentration during myocardial ischemia by spin label oximetry has been limited by ischemia-induced reduction of the neutral, water-soluble nitroxide TEMPONE. We have overcome this problem by encapsulating the nitroxides. Isolated immature (7-10 d old) rabbit hearts (n = 8) were perfused aerobically within the cavity of a loop gap resonator with bicarbonate buffer containing an oxygen-sensitive, lipid-soluble nitroxide (14N-TEMPO laurate in FC-43 perfluorocarbon micelles) and a much less oxygen-sensitive and positively charged nitroxide (15N-TEMPO choline in multilamellar vesicles) as an internal standard. The ratio of the ESR signal amplitudes of these nitroxides was used as a sensitive index of oxygen concentration. Sequestration of the nitroxides decreased their reduction rate by ascorbate in comparison with nonsequestered nitroxides. Hearts were subjected to 60 min of global no-flow ischemia at 20 degrees C. Extracellular oxygen content (mean +/- SD) during aerobic perfusion was 1195 +/- 55 mumol/liter. The electron spin resonance signal from TEMPO laurate increased with the onset and progression of ischemia, consistent with a decrease in extracellular oxygen, while the signal for TEMPO choline was relatively unchanged. Extracellular oxygen content after 40 and 60 min of ischemia was reduced to 393 +/- 27 mumol/liter (p < .05) and 61 +/- 5 mumol/liter (p < .05), respectively. We conclude that spin-label oximetry can directly and precisely measure myocardial oxygen consumption at constant temperature during ischemia in the intact heart. | lld:pubmed |
| pubmed-article:8958135 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8958135 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8958135 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8958135 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8958135 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8958135 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8958135 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8958135 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8958135 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8958135 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8958135 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8958135 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8958135 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8958135 | pubmed:issn | 0891-5849 | lld:pubmed |
| pubmed-article:8958135 | pubmed:author | pubmed-author:KalyanaramanB... | lld:pubmed |
| pubmed-article:8958135 | pubmed:author | pubmed-author:BakerJ EJE | lld:pubmed |
| pubmed-article:8958135 | pubmed:author | pubmed-author:FronciszWW | lld:pubmed |
| pubmed-article:8958135 | pubmed:author | pubmed-author:JosephJJ | lld:pubmed |
| pubmed-article:8958135 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8958135 | pubmed:volume | 22 | lld:pubmed |
| pubmed-article:8958135 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8958135 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8958135 | pubmed:pagination | 109-15 | lld:pubmed |
| pubmed-article:8958135 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8958135 | pubmed:meshHeading | pubmed-meshheading:8958135-... | lld:pubmed |
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| pubmed-article:8958135 | pubmed:meshHeading | pubmed-meshheading:8958135-... | lld:pubmed |
| pubmed-article:8958135 | pubmed:meshHeading | pubmed-meshheading:8958135-... | lld:pubmed |
| pubmed-article:8958135 | pubmed:meshHeading | pubmed-meshheading:8958135-... | lld:pubmed |
| pubmed-article:8958135 | pubmed:meshHeading | pubmed-meshheading:8958135-... | lld:pubmed |
| pubmed-article:8958135 | pubmed:meshHeading | pubmed-meshheading:8958135-... | lld:pubmed |
| pubmed-article:8958135 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:8958135 | pubmed:articleTitle | Spin label oximetry to assess extracellular oxygen during myocardial ischemia. | lld:pubmed |
| pubmed-article:8958135 | pubmed:affiliation | Department of Cardiothoracic Surgery, Medical College of Wisconsin, Milwaukee 53226, USA. | lld:pubmed |
| pubmed-article:8958135 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8958135 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:8958135 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8958135 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8958135 | lld:pubmed |
