| pubmed-article:8955146 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8955146 | lifeskim:mentions | umls-concept:C0014644 | lld:lifeskim |
| pubmed-article:8955146 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:8955146 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:8955146 | lifeskim:mentions | umls-concept:C0017428 | lld:lifeskim |
| pubmed-article:8955146 | lifeskim:mentions | umls-concept:C1167117 | lld:lifeskim |
| pubmed-article:8955146 | lifeskim:mentions | umls-concept:C1550009 | lld:lifeskim |
| pubmed-article:8955146 | lifeskim:mentions | umls-concept:C0971226 | lld:lifeskim |
| pubmed-article:8955146 | pubmed:issue | 51 | lld:pubmed |
| pubmed-article:8955146 | pubmed:dateCreated | 1997-1-23 | lld:pubmed |
| pubmed-article:8955146 | pubmed:abstractText | We have previously shown that replication forks stall at a family of repeated sequences (FR) within the Epstein-Barr virus latent origin of replication oriP, both in a small plasmid and in the intact Epstein-Barr virus genome. Each of the 20 repeated sequences within the FR contains a binding site for Epstein-Barr nuclear antigen 1 (EBNA-1), the only viral protein required for latent replication. We showed that the EBNA-1 protein enhances the accumulation of paused replication forks at the FR. In this study, we have investigated a series of truncated EBNA-1 proteins to determine the portion of the EBNA-1 protein that is responsible for pausing of forks at the FR. Two-dimensional agarose gel electrophoresis was performed on the products of in vitro replication reactions in the presence of full-length EBNA-1 or proteins with various deletions to assess the extent of fork pausing at the FR. We conclude that a portion of the DNA binding domain is important for fork pausing. We also present evidence indicating that phosphorylation of the EBNA-1 protein or EBNA-1-truncated derivatives is not essential for pausing. To investigate the mechanism of EBNA-1-mediated pausing of replication forks, we asked whether EBNA-1 could inhibit the DNA unwinding activity of replicative helicases. We found that EBNA-1, when bound to the FR, inhibits DNA unwinding in vitro by SV40 T antigen and Escherichia coli dnaB helicases in an orientation-independent manner. | lld:pubmed |
| pubmed-article:8955146 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8955146 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8955146 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8955146 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8955146 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8955146 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8955146 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8955146 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8955146 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8955146 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8955146 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:8955146 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:8955146 | pubmed:author | pubmed-author:SchildkrautC... | lld:pubmed |
| pubmed-article:8955146 | pubmed:author | pubmed-author:FrappierLL | lld:pubmed |
| pubmed-article:8955146 | pubmed:author | pubmed-author:ErmakovaO VOV | lld:pubmed |
| pubmed-article:8955146 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8955146 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:8955146 | pubmed:volume | 271 | lld:pubmed |
| pubmed-article:8955146 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8955146 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8955146 | pubmed:pagination | 33009-17 | lld:pubmed |
| pubmed-article:8955146 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8955146 | pubmed:meshHeading | pubmed-meshheading:8955146-... | lld:pubmed |
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| pubmed-article:8955146 | pubmed:meshHeading | pubmed-meshheading:8955146-... | lld:pubmed |
| pubmed-article:8955146 | pubmed:meshHeading | pubmed-meshheading:8955146-... | lld:pubmed |
| pubmed-article:8955146 | pubmed:meshHeading | pubmed-meshheading:8955146-... | lld:pubmed |
| pubmed-article:8955146 | pubmed:meshHeading | pubmed-meshheading:8955146-... | lld:pubmed |
| pubmed-article:8955146 | pubmed:meshHeading | pubmed-meshheading:8955146-... | lld:pubmed |
| pubmed-article:8955146 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8955146 | pubmed:articleTitle | Role of the EBNA-1 protein in pausing of replication forks in the Epstein-Barr virus genome. | lld:pubmed |
| pubmed-article:8955146 | pubmed:affiliation | Department of Cell Biology, Albert Einstein College of Medicine, New York, New York 10461, USA. | lld:pubmed |
| pubmed-article:8955146 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8955146 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8955146 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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