| pubmed-article:8938018 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8938018 | lifeskim:mentions | umls-concept:C1512505 | lld:lifeskim |
| pubmed-article:8938018 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:8938018 | lifeskim:mentions | umls-concept:C0449560 | lld:lifeskim |
| pubmed-article:8938018 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:8938018 | pubmed:issue | 4-5 | lld:pubmed |
| pubmed-article:8938018 | pubmed:dateCreated | 1997-2-27 | lld:pubmed |
| pubmed-article:8938018 | pubmed:abstractText | beta-adrenergic receptors (beta-ARs) were identified in CG-5 breast cancer cells using a radiometric assay. The total beta-AR concentration was measured using the highly potent beta-adrenergic antagonist (-)[3H]CGP 12177, and the densities of beta-AR subtypes were discriminated in the presence of highly selective unlabelled ligands (CGP 20712A and ICI 118551). Scatchard analysis revealed good linearity (r > 0.95) and Kd values (0.05-1 nM) indicated the presence of high affinity binding sites in CG-5 cell membranes. beta 2-AR concentrations (74%) were significantly (P < 0.05) higher than beta 1-AR concentrations (36%). Displacement studies indicated that tested adrenergic agonists displaced (-) [3H]CGP 12177 from its specific binding sites in the order of potency (-)isoproterenol > (+/-)clenbuterol > (-)adrenaline > (+/-)dobutamine > > (-)noradrenaline, whereas beta-adrenergic antagonists inhibited the binding in the following order of potency: (-)propranolol > > ICI 118 551 > > CGP 20712A. The functionality of beta-ARs identified in CG-5 cell membranes was demonstrated by the significant increase in cAMP production induced by different concentrations of isoproterenol vs unstimulated cells (control). The pathophysiological role of beta-ARs in breast cancer cells is still undefined, but their presence suggests the possible adrenergic regulation of some cellular activities such as proliferation and/or differentiation. | lld:pubmed |
| pubmed-article:8938018 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8938018 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8938018 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8938018 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8938018 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8938018 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8938018 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8938018 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8938018 | pubmed:issn | 1043-6618 | lld:pubmed |
| pubmed-article:8938018 | pubmed:author | pubmed-author:Di CarloFF | lld:pubmed |
| pubmed-article:8938018 | pubmed:author | pubmed-author:NovelliAA | lld:pubmed |
| pubmed-article:8938018 | pubmed:author | pubmed-author:GirardiCC | lld:pubmed |
| pubmed-article:8938018 | pubmed:author | pubmed-author:BadinoG RGR | lld:pubmed |
| pubmed-article:8938018 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8938018 | pubmed:volume | 33 | lld:pubmed |
| pubmed-article:8938018 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8938018 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8938018 | pubmed:pagination | 255-60 | lld:pubmed |
| pubmed-article:8938018 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8938018 | pubmed:meshHeading | pubmed-meshheading:8938018-... | lld:pubmed |
| pubmed-article:8938018 | pubmed:meshHeading | pubmed-meshheading:8938018-... | lld:pubmed |
| pubmed-article:8938018 | pubmed:meshHeading | pubmed-meshheading:8938018-... | lld:pubmed |
| pubmed-article:8938018 | pubmed:meshHeading | pubmed-meshheading:8938018-... | lld:pubmed |
| pubmed-article:8938018 | pubmed:meshHeading | pubmed-meshheading:8938018-... | lld:pubmed |
| pubmed-article:8938018 | pubmed:meshHeading | pubmed-meshheading:8938018-... | lld:pubmed |
| pubmed-article:8938018 | pubmed:meshHeading | pubmed-meshheading:8938018-... | lld:pubmed |
| pubmed-article:8938018 | pubmed:articleTitle | Evidence for functional beta-adrenoceptor subtypes in CG-5 breast cancer cell. | lld:pubmed |
| pubmed-article:8938018 | pubmed:affiliation | Department of Animal Pathology, University of Torino, Italy. | lld:pubmed |
| pubmed-article:8938018 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8938018 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8938018 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8938018 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8938018 | lld:pubmed |
