| pubmed-article:8935658 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8935658 | lifeskim:mentions | umls-concept:C0315191 | lld:lifeskim |
| pubmed-article:8935658 | lifeskim:mentions | umls-concept:C0086022 | lld:lifeskim |
| pubmed-article:8935658 | lifeskim:mentions | umls-concept:C1527177 | lld:lifeskim |
| pubmed-article:8935658 | lifeskim:mentions | umls-concept:C0441513 | lld:lifeskim |
| pubmed-article:8935658 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8935658 | pubmed:dateCreated | 1996-12-31 | lld:pubmed |
| pubmed-article:8935658 | pubmed:abstractText | Two plasmid cloning vectors (pULMJ55 and pULMJ95) were constructed for Brevibacterium lactofermentum using the origin of replication of the endogenous plasmid pBL1. Plasmid pULMJ55 is a replacement vector with transcriptional terminators from the B. lactofermentum trp operon flanking the BglII cloning sites. Religation of the BglII digested vector without insert creates a 376 bp perfect palindrome that is not tolerated in B. lactofermentum, giving positive selection for recombinant plasmids with inserts. Plasmid pULMJ95 contains the promoter-less alpha-amylase gene from Streptomyces griseus downstream of the trp terminator and is particularly suitable for the detection of promoters which are activated late during the growth phase. alpha-Amylase is secreted and its activity can be detected using simple plate tests. | lld:pubmed |
| pubmed-article:8935658 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8935658 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8935658 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8935658 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8935658 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8935658 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8935658 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8935658 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:8935658 | pubmed:issn | 0378-1097 | lld:pubmed |
| pubmed-article:8935658 | pubmed:author | pubmed-author:MartínJ FJF | lld:pubmed |
| pubmed-article:8935658 | pubmed:author | pubmed-author:GilJ AJA | lld:pubmed |
| pubmed-article:8935658 | pubmed:author | pubmed-author:CadenasR FRF | lld:pubmed |
| pubmed-article:8935658 | pubmed:author | pubmed-author:Fernández-Gon... | lld:pubmed |
| pubmed-article:8935658 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8935658 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:8935658 | pubmed:volume | 137 | lld:pubmed |
| pubmed-article:8935658 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8935658 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8935658 | pubmed:pagination | 63-8 | lld:pubmed |
| pubmed-article:8935658 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:8935658 | pubmed:meshHeading | pubmed-meshheading:8935658-... | lld:pubmed |
| pubmed-article:8935658 | pubmed:meshHeading | pubmed-meshheading:8935658-... | lld:pubmed |
| pubmed-article:8935658 | pubmed:meshHeading | pubmed-meshheading:8935658-... | lld:pubmed |
| pubmed-article:8935658 | pubmed:meshHeading | pubmed-meshheading:8935658-... | lld:pubmed |
| pubmed-article:8935658 | pubmed:meshHeading | pubmed-meshheading:8935658-... | lld:pubmed |
| pubmed-article:8935658 | pubmed:meshHeading | pubmed-meshheading:8935658-... | lld:pubmed |
| pubmed-article:8935658 | pubmed:meshHeading | pubmed-meshheading:8935658-... | lld:pubmed |
| pubmed-article:8935658 | pubmed:meshHeading | pubmed-meshheading:8935658-... | lld:pubmed |
| pubmed-article:8935658 | pubmed:meshHeading | pubmed-meshheading:8935658-... | lld:pubmed |
| pubmed-article:8935658 | pubmed:meshHeading | pubmed-meshheading:8935658-... | lld:pubmed |
| pubmed-article:8935658 | pubmed:meshHeading | pubmed-meshheading:8935658-... | lld:pubmed |
| pubmed-article:8935658 | pubmed:meshHeading | pubmed-meshheading:8935658-... | lld:pubmed |
| pubmed-article:8935658 | pubmed:meshHeading | pubmed-meshheading:8935658-... | lld:pubmed |
| pubmed-article:8935658 | pubmed:meshHeading | pubmed-meshheading:8935658-... | lld:pubmed |
| pubmed-article:8935658 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8935658 | pubmed:articleTitle | Construction of new cloning vectors for Brevibacterium lactofermentum. | lld:pubmed |
| pubmed-article:8935658 | pubmed:affiliation | Departamento de Ecología, Genética y Microbiología, Universidad de León, Spain. | lld:pubmed |
| pubmed-article:8935658 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8935658 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
